New Multi-Walled carbon nanotube of industrial interest induce cell death in murine fibroblast cells

The search for new nanomaterials has brought to the multifactorial industry several opportunities for use and applications for existing materials. Carbon nanotubes (CNT), for example, present excellent properties which allow us to assume a series of applications, however there is concern in the industrial scope about possible adverse health effects related to constant exposure for inhalation or direct skin contact. Thus, using cell models is the fastest and safest way to assess the effects of a new material. The aim of this study was to investigate the cytotoxic profile in LA9 murine fibroblast lineage, of a new multi-walled carbon nanotube (MWCNT) that was functionalized with tetraethylenepentamine (TEPA) to obtain better physical-chemical characteristics for industrial use. The modifications presented in the CNT cause concern, as they can change its initial characteristics, making this nanomaterial harmful. HR-TEM, FE-SEM and zeta potential were used for the characterization. Cytotoxicity and cell proliferation tests, oxidative and nitrosative stress analyzes and inflammatory cytokine assay (TNF-α) were performed. The main findings demonstrated a reduction in cell viability, increased release of intracellular ROS, accompanied by an increase in TNF-α, indicating an important inflammatory profile. Confirmation of the data was performed by flow cytometry and ImageXpress with apoptosis/necrosis markers. These data provide initial evidence that OCNT-TEPA has a cytotoxic profile dependent on the concentration of LA9 fibroblasts, since there was an increase in free radicals, inflammation induction and cell death, suggesting that continuous exposure to this nanoparticle can cause damage to different tissues in the organism.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:31

Enthalten in:

Toxicology mechanisms and methods - 31(2021), 7 vom: 02. Sept., Seite 517-530

Sprache:

Englisch

Beteiligte Personen:

de Godoy, Krissia Franco [VerfasserIn]
de Almeida Rodolpho, Joice Margareth [VerfasserIn]
Brassolatti, Patricia [VerfasserIn]
de Lima Fragelli, Bruna Dias [VerfasserIn]
de Castro, Cynthia Aparecida [VerfasserIn]
Assis, Marcelo [VerfasserIn]
Cancino Bernardi, Juliana [VerfasserIn]
de Oliveira Correia, Ricardo [VerfasserIn]
Albuquerque, Yulli Roxenne [VerfasserIn]
Speglich, Carlos [VerfasserIn]
Longo, Elson [VerfasserIn]
de Freitas Anibal, Fernanda [VerfasserIn]

Links:

Volltext

Themen:

Apoptosis/necrosis
Cytotoxicity
Inflammation
Journal Article
Multi-walled carbon nanotube
Murine fibroblast cells
Nanomaterials
Nanoparticles
Nanotubes, Carbon

Anmerkungen:

Date Completed 19.11.2021

Date Revised 19.11.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1080/15376516.2021.1930311

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM325496447