Preparation and evaluation of virus-like particle vaccine against H3N8 subtype equine influenza
Copyright © 2021 Elsevier Ltd. All rights reserved..
To prevent and control H3N8 subtype equine influenza, we prepared virus-like particles (VLPs) comprising the HA, NA and M1 proteins of H3N8 equine influenza virus (EIV) through the insect cell-baculovirus expression system. The results of Western blot and hemagglutination analyses demonstrated that the constructed VLPs comprising HA, NA and M1 proteins have good hemagglutination activity. Immunoelectron microscope revealed that the VLPs share similar morphology and structure with natural virus particles. The hyperimmune serum from horses immunized with the VLPs were injected into mice by means of artificial passive immunization and then challenge, or challenge following by injecting hyperimmune serum. The results showed that the equine hyperimmune serum has good preventive and therapeutic efficacy against the infection of H3N8 EIV. The study provides a technical foundation for the development of H3N8 EIV VLP vaccine.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:157 |
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| Enthalten in: |
Microbial pathogenesis - 157(2021) vom: 05. Aug., Seite 104885 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Pengju [VerfasserIn] |
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| Links: |
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| Themen: |
Antibodies, Viral |
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| Anmerkungen: |
Date Completed 21.06.2021 Date Revised 21.06.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.micpath.2021.104885 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM325429480 |
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| 520 | |a Copyright © 2021 Elsevier Ltd. All rights reserved. | ||
| 520 | |a To prevent and control H3N8 subtype equine influenza, we prepared virus-like particles (VLPs) comprising the HA, NA and M1 proteins of H3N8 equine influenza virus (EIV) through the insect cell-baculovirus expression system. The results of Western blot and hemagglutination analyses demonstrated that the constructed VLPs comprising HA, NA and M1 proteins have good hemagglutination activity. Immunoelectron microscope revealed that the VLPs share similar morphology and structure with natural virus particles. The hyperimmune serum from horses immunized with the VLPs were injected into mice by means of artificial passive immunization and then challenge, or challenge following by injecting hyperimmune serum. The results showed that the equine hyperimmune serum has good preventive and therapeutic efficacy against the infection of H3N8 EIV. The study provides a technical foundation for the development of H3N8 EIV VLP vaccine | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Equine influenza | |
| 650 | 4 | |a H3N8 subtype | |
| 650 | 4 | |a Hyperimmune serum | |
| 650 | 4 | |a Preventive and therapeutic efficacy | |
| 650 | 4 | |a Virus-like particles | |
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| 650 | 7 | |a Vaccines, Virus-Like Particle |2 NLM | |
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| 700 | 1 | |a Ling, Mengmeng |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xintao |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Weixia |e verfasserin |4 aut | |
| 700 | 1 | |a Cong, Yanlong |e verfasserin |4 aut | |
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