Sex-Related Embryotoxicity of Pulp Mill Effluent Extracts in Medaka (Oryzias latipes) Female Leucophore-free FLFII Strain

© 2021 SETAC..

The aim of the present study was to evaluate the effects of exposure to Chilean pulp mill effluent extracts on developing postfertilized medaka embryos before and after sex definition relative to sex steroids (testosterone and 17beta-estradiol) and a wood phytoestrogen (beta-sitosterol). Our study included 2 waterborne semichronic exposure experiments, using a 24-h post fertilization (hpf) unknown-sex FLFII (female leucophore free) group and a second 72-hpf FLFII phenotypic sex-identified group (male autofluorescence leucophore) strain of medaka embryos. Chronic exposure of both FLFII strain embryo groups showed similar delay in time to hatch and decreased hatchability. Teratogenic responses such as vertebral malformation (fusion, incomplete formation, and lack of vertebral formation process) and pericardial edema were observed in both experiments, with a high percentage related to FLFII fluorescent leucophore-identified males. In addition, high mortality associated with severe malformations was observed in male and female embryos exposed to testosterone. Our research has demonstrated that exposure to Chilean mill effluent extracts caused severe male medaka embryotoxicity (in postfertilized embryos) before and after sex definition and, irrespective of the experimental group and effluent treatment, suggests partial removal following secondary treatment. Furthermore, differences in the severity and type of teratogenic effects with previous experiments (d-rR medaka strain), are associated with the unique phenotypes of this medaka mutant strain. Environ Toxicol Chem 2021;40:2297-2305. © 2021 SETAC.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:40

Enthalten in:

Environmental toxicology and chemistry - 40(2021), 8 vom: 16. Aug., Seite 2297-2305

Sprache:

Englisch

Beteiligte Personen:

Orrego, Rodrigo [VerfasserIn]
Guchardi, John [VerfasserIn]
Beyger, Lindsay [VerfasserIn]
Barra, Ricardo [VerfasserIn]
Hewitt, L Mark [VerfasserIn]
Holdway, Douglas [VerfasserIn]

Links:

Volltext

Themen:

3XMK78S47O
4TI98Z838E
Embryos
Estradiol
Journal Article
Medaka FLFII
Plant Extracts
Pulp mill extracts
Research Support, Non-U.S. Gov't
Teratogenic
Testosterone

Anmerkungen:

Date Completed 14.04.2022

Date Revised 14.04.2022

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1002/etc.5115

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM325297606