Dendrimer-assisted boronate affinity cellulose foams for the efficient and selective separation of glycoproteins
Copyright © 2021 Elsevier Ltd. All rights reserved..
Surfaces engineered to identify and enrich glycoproteins are of considerable interest in the diagnostic and detection fields. A boronate affinity (BA) material was proposed as a potential candidate for the isolation of glycoproteins. However, this material has the disadvantages of low efficiency and non-degradability. Herein, a novel dendrimer-amplified BA cellulose foam (PEI-PBA-CF) was fabricated via a mild two-step approach. The as-prepared PEI-PBA-CF exhibited a rapid adsorption equilibrium rate (within 60 min) and outstanding adsorption capacity for horseradish peroxidase (537.4 mg g-1) and ovalbumin (495.5 mg g-1). Furthermore, competitive adsorption experiments demonstrated that PEI-PBA-CF could achieve selective separation and purification of glycoproteins from complex biological samples due to the synergistic effect of the improved BA capacity by the dendrimer and the well-interconnected porous structure of the biomass matrix. Consequently, these cellulose foams might present new application opportunities in analytical and biomedical fields.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2021 |
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Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:265 |
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Enthalten in: |
Carbohydrate polymers - 265(2021) vom: 01. Aug., Seite 118082 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Yao, Xue [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 04.10.2021 Date Revised 04.10.2021 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.carbpol.2021.118082 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM325185123 |
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520 | |a Copyright © 2021 Elsevier Ltd. All rights reserved. | ||
520 | |a Surfaces engineered to identify and enrich glycoproteins are of considerable interest in the diagnostic and detection fields. A boronate affinity (BA) material was proposed as a potential candidate for the isolation of glycoproteins. However, this material has the disadvantages of low efficiency and non-degradability. Herein, a novel dendrimer-amplified BA cellulose foam (PEI-PBA-CF) was fabricated via a mild two-step approach. The as-prepared PEI-PBA-CF exhibited a rapid adsorption equilibrium rate (within 60 min) and outstanding adsorption capacity for horseradish peroxidase (537.4 mg g-1) and ovalbumin (495.5 mg g-1). Furthermore, competitive adsorption experiments demonstrated that PEI-PBA-CF could achieve selective separation and purification of glycoproteins from complex biological samples due to the synergistic effect of the improved BA capacity by the dendrimer and the well-interconnected porous structure of the biomass matrix. Consequently, these cellulose foams might present new application opportunities in analytical and biomedical fields | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Boronic acid | |
650 | 4 | |a Branched polyethyleneimine | |
650 | 4 | |a Cellulose foam | |
650 | 4 | |a Enrichment | |
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650 | 7 | |a Glycoproteins |2 NLM | |
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700 | 1 | |a Du, Min |e verfasserin |4 aut | |
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