Dendrimer-assisted boronate affinity cellulose foams for the efficient and selective separation of glycoproteins

Copyright © 2021 Elsevier Ltd. All rights reserved..

Surfaces engineered to identify and enrich glycoproteins are of considerable interest in the diagnostic and detection fields. A boronate affinity (BA) material was proposed as a potential candidate for the isolation of glycoproteins. However, this material has the disadvantages of low efficiency and non-degradability. Herein, a novel dendrimer-amplified BA cellulose foam (PEI-PBA-CF) was fabricated via a mild two-step approach. The as-prepared PEI-PBA-CF exhibited a rapid adsorption equilibrium rate (within 60 min) and outstanding adsorption capacity for horseradish peroxidase (537.4 mg g-1) and ovalbumin (495.5 mg g-1). Furthermore, competitive adsorption experiments demonstrated that PEI-PBA-CF could achieve selective separation and purification of glycoproteins from complex biological samples due to the synergistic effect of the improved BA capacity by the dendrimer and the well-interconnected porous structure of the biomass matrix. Consequently, these cellulose foams might present new application opportunities in analytical and biomedical fields.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:265

Enthalten in:

Carbohydrate polymers - 265(2021) vom: 01. Aug., Seite 118082

Sprache:

Englisch

Beteiligte Personen:

Yao, Xue [VerfasserIn]
Zhang, Sufeng [VerfasserIn]
Qian, Liwei [VerfasserIn]
Du, Min [VerfasserIn]

Links:

Volltext

Themen:

9002-98-6
9004-34-6
9006-59-1
Boronic Acids
Boronic acid
Branched polyethyleneimine
Cellulose
Cellulose foam
Dendrimers
EC 1.11.1.-
Enrichment
Glycoprotein
Glycoproteins
Horseradish Peroxidase
Isolation
Journal Article
Ovalbumin
Polyethyleneimine
Schiff Bases

Anmerkungen:

Date Completed 04.10.2021

Date Revised 04.10.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.carbpol.2021.118082

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM325185123