Boron deficiency-induced root growth inhibition is mediated by brassinosteroid signalling regulation in Arabidopsis
© 2021 Society for Experimental Biology and John Wiley & Sons Ltd..
Brassinosteroids (BRs) are pivotal phytohormones involved in the control of root development. Boron (B) is an essential micronutrient for plants, and root growth is rapidly inhibited under B deficiency conditions. However, the mechanisms underlying this inhibition are still unclear. Here, we identified BR-related processes underlying B deficiency at the physiological, genetic, molecular/cell biological and transcriptomic levels and found strong evidence that B deficiency can affect BR biosynthesis and signalling, thereby altering root growth. RNA sequencing analysis revealed strong co-regulation between BR-regulated genes and B deficiency-responsive genes. We found that the BR receptor mutants bri1-119 and bri1-301 were more insensitive to decreased B supply, and the gain-of-function mutants bes1-D and pBZR1-bzr1-D exhibited insensitivity to low-B stress. Under B deficiency conditions, exogenous 24-epibrassinolide rescued the inhibition of root growth, and application of the BR biosynthesis inhibitor brassinazole exacerbated this inhibitory effect. The nuclear-localised signal of BES1 was reduced under low-B conditions compared with B sufficiency conditions. We further found that B deficiency hindered the accumulation of brassinolide to downregulate BR signalling and modulate root elongation, which may occur through a reduction in BR6ox1 and BR6ox2 mRNA levels. Taken together, our results reveal a role of BR signalling in root elongation under B deficiency.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:107 |
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| Enthalten in: |
The Plant journal : for cell and molecular biology - 107(2021), 2 vom: 08. Juli, Seite 564-578 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Cheng [VerfasserIn] |
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| Links: |
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| Themen: |
Arabidopsis |
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| Anmerkungen: |
Date Completed 29.11.2021 Date Revised 29.11.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/tpj.15311 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM325157189 |
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| 245 | 1 | 0 | |a Boron deficiency-induced root growth inhibition is mediated by brassinosteroid signalling regulation in Arabidopsis |
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| 520 | |a © 2021 Society for Experimental Biology and John Wiley & Sons Ltd. | ||
| 520 | |a Brassinosteroids (BRs) are pivotal phytohormones involved in the control of root development. Boron (B) is an essential micronutrient for plants, and root growth is rapidly inhibited under B deficiency conditions. However, the mechanisms underlying this inhibition are still unclear. Here, we identified BR-related processes underlying B deficiency at the physiological, genetic, molecular/cell biological and transcriptomic levels and found strong evidence that B deficiency can affect BR biosynthesis and signalling, thereby altering root growth. RNA sequencing analysis revealed strong co-regulation between BR-regulated genes and B deficiency-responsive genes. We found that the BR receptor mutants bri1-119 and bri1-301 were more insensitive to decreased B supply, and the gain-of-function mutants bes1-D and pBZR1-bzr1-D exhibited insensitivity to low-B stress. Under B deficiency conditions, exogenous 24-epibrassinolide rescued the inhibition of root growth, and application of the BR biosynthesis inhibitor brassinazole exacerbated this inhibitory effect. The nuclear-localised signal of BES1 was reduced under low-B conditions compared with B sufficiency conditions. We further found that B deficiency hindered the accumulation of brassinolide to downregulate BR signalling and modulate root elongation, which may occur through a reduction in BR6ox1 and BR6ox2 mRNA levels. Taken together, our results reveal a role of BR signalling in root elongation under B deficiency | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a BR6ox1 | |
| 650 | 4 | |a BR6ox2 | |
| 650 | 4 | |a Arabidopsis | |
| 650 | 4 | |a boron deficiency | |
| 650 | 4 | |a brassinolide | |
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| 700 | 1 | |a He, Mingliang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Sheliang |e verfasserin |4 aut | |
| 700 | 1 | |a Chu, Liuyang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Chuang |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Ningmei |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Guangda |e verfasserin |4 aut | |
| 700 | 1 | |a Cai, Hongmei |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Fangsen |e verfasserin |4 aut | |
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