Overexpressing microRNA-203 alleviates myocardial infarction via interacting with long non-coding RNA MIAT and mitochondrial coupling factor 6

Myocardial infarction (MI) is one of the leading causes of high mortality worldwide. Long non-coding RNA myocardial infarction associated transcript (MIAT) and mitochondrial coupling factor 6 (CF6) aggravate MI. This study aimed to elucidate whether miR-203 interacted with MIAT and CF6 in MI. Results revealed that MIAT and CF6 expressions were upregulated and that miR-203 was downregulated in mouse myocardial tissues after MI, as well as in hypoxic mouse cardiomyocytes. The overexpression of MIAT in mouse cardiomyocytes raised CF6 expression, whereas the knockdown of MIAT had the opposite effect. Mechanistically, the luciferase reporter and RNA pull-down assays corroborated the binding between miR-203 and CF6 3'UTR and between miR-203 and MIAT. The simultaneous overexpression of miR-203 and MIAT restored the reduction of CF6 caused by miR-203 overexpression alone, and the overexpression of miR-203 diminished the percentage of infarct area and the apoptosis of cardiomyocytes in vivo. Our findings corroborate that overexpressing miR-203 alleviates MI via interacting with MIAT and CF6.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:44

Enthalten in:

Archives of pharmacal research - 44(2021), 5 vom: 03. Mai, Seite 525-535

Sprache:

Englisch

Beteiligte Personen:

Wang, Fan [VerfasserIn]
Yu, Renliang [VerfasserIn]
Wen, Shengnan [VerfasserIn]
Yin, Jie [VerfasserIn]
Shi, Yugen [VerfasserIn]
Hu, Hesheng [VerfasserIn]
Yan, Suhua [VerfasserIn]

Links:

Volltext

Themen:

Apoptosis
CF6
EC 3.6.1.-
EC 3.6.3.-
F(6) ATPase
Journal Article
MIAT
MIRN203 microRNA, mouse
MiR-203
Miat long non-coding RNA
MicroRNAs
Mitochondrial Proton-Translocating ATPases
Myocardial infarction
Oxidative Phosphorylation Coupling Factors
RNA, Long Noncoding

Anmerkungen:

Date Completed 03.12.2021

Date Revised 14.12.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1007/s12272-021-01324-8

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM324952481