Manipulating a Single Transcription Factor, Ant1, Promotes Anthocyanin Accumulation in Barley Grains
Barley has abundant anthocyanin-rich accessions, which renders it an ideal model to investigate the regulatory mechanism of anthocyanin biosynthesis. This study functionally characterized two transcription factors: Ant1 and Ant2. Sequence alignment showed that the coding sequences of Ant1 and Ant2 are conserved among 11 colored hulless barley and noncolored barley varieties. The expression profiles of Ant1 and Ant2 were divergent between species, and significantly higher expression was found in two colored Qingke accessions. The co-expression of Ant1 and Ant2 resulted in purple pigmentation in transient transformation systems via the promotion of the transcription of four structural genes. Ant1 interacted with Ant2, and overexpression of Ant1 activated the transcription of Ant2. Moreover, overexpression of Ant1 led to anthocyanin accumulation in the pericarp and aleurone layer of transgenic barley grains. Overall, our results suggest that anthocyanin-enriched barley grains can be produced by manipulating Ant1 expression.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:69 |
|---|---|
| Enthalten in: |
Journal of agricultural and food chemistry - 69(2021), 18 vom: 12. Mai, Seite 5306-5317 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zhou, Chenlu [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Anthocyanin biosynthesis |
|---|
| Anmerkungen: |
Date Completed 13.05.2021 Date Revised 13.05.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1021/acs.jafc.0c08147 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM324616341 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM324616341 | ||
| 003 | DE-627 | ||
| 005 | 20231225190712.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/acs.jafc.0c08147 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1082.xml |
| 035 | |a (DE-627)NLM324616341 | ||
| 035 | |a (NLM)33908247 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zhou, Chenlu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Manipulating a Single Transcription Factor, Ant1, Promotes Anthocyanin Accumulation in Barley Grains |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 13.05.2021 | ||
| 500 | |a Date Revised 13.05.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Barley has abundant anthocyanin-rich accessions, which renders it an ideal model to investigate the regulatory mechanism of anthocyanin biosynthesis. This study functionally characterized two transcription factors: Ant1 and Ant2. Sequence alignment showed that the coding sequences of Ant1 and Ant2 are conserved among 11 colored hulless barley and noncolored barley varieties. The expression profiles of Ant1 and Ant2 were divergent between species, and significantly higher expression was found in two colored Qingke accessions. The co-expression of Ant1 and Ant2 resulted in purple pigmentation in transient transformation systems via the promotion of the transcription of four structural genes. Ant1 interacted with Ant2, and overexpression of Ant1 activated the transcription of Ant2. Moreover, overexpression of Ant1 led to anthocyanin accumulation in the pericarp and aleurone layer of transgenic barley grains. Overall, our results suggest that anthocyanin-enriched barley grains can be produced by manipulating Ant1 expression | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a MYB/bHLH/WD40 complex | |
| 650 | 4 | |a anthocyanin biosynthesis | |
| 650 | 4 | |a barley | |
| 650 | 4 | |a transcriptional regulation | |
| 650 | 7 | |a Anthocyanins |2 NLM | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 650 | 7 | |a Transcription Factors |2 NLM | |
| 700 | 1 | |a Zeng, Zhanghui |e verfasserin |4 aut | |
| 700 | 1 | |a Suo, Jingqi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xipu |e verfasserin |4 aut | |
| 700 | 1 | |a Bian, Hongwu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Junhui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Muyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Ning |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of agricultural and food chemistry |d 1967 |g 69(2021), 18 vom: 12. Mai, Seite 5306-5317 |w (DE-627)NLM000005665 |x 1520-5118 |7 nnns |
| 773 | 1 | 8 | |g volume:69 |g year:2021 |g number:18 |g day:12 |g month:05 |g pages:5306-5317 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1021/acs.jafc.0c08147 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 69 |j 2021 |e 18 |b 12 |c 05 |h 5306-5317 | ||