Manipulating and tracking single hepatocyte behavior during mouse liver regeneration by performing hydrodynamic tail vein injection

© 2021 The Author(s)..

Studies to identify genes relevant to mammalian hepatocyte biology in vivo are largely carried out using germline genetic-engineering approaches, which can be costly and time-consuming. We describe hydrodynamic tail vein injection as an alternative approach to introduce genetic elements into hepatocytes. Transfected hepatocytes can then be traced with a GFP reporter enabling the use of immunohistochemistry and FACS sorting to examine the changes in hepatocyte gene expression and proliferation during liver regeneration induced by 2/3 partial hepatectomy (PH). For complete details on the use and execution of this protocol, please refer to Wang et al. (2019).

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:2

Enthalten in:

STAR protocols - 2(2021), 2 vom: 18. Juni, Seite 100440

Sprache:

Englisch

Beteiligte Personen:

Wang, Shuang [VerfasserIn]
Ruiz de Galarreta, Marina [VerfasserIn]
Sadler, Kirsten C [VerfasserIn]
Lujambio, Amaia [VerfasserIn]

Links:

Volltext

Themen:

Cell Biology
Flow Cytometry/Mass Cytometry
Journal Article
Metabolism
Microscopy
Model Organisms
Molecular Biology
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 21.03.2022

Date Revised 21.03.2022

published: Electronic-eCollection

Citation Status MEDLINE

doi:

10.1016/j.xpro.2021.100440

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM32452563X