Ecotin and LamB in Escherichia coli influence the susceptibility to Type VI secretion-mediated interbacterial competition and killing by Vibrio cholerae
Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved..
BACKGROUND: A prevailing action of the Type VI secretion system (T6SS) in several Gram-negative bacterial species is inter-bacterial competition. In the past several years, many effectors of T6SS were identified in different bacterial species and their involvement in inter-bacterial interactions were described. However, possible defence mechanisms against T6SS attack among prey bacteria were not well clarified yet.
METHODS: Escherichia coli was assessed for susceptibility to T6SS-mediated killing by Vibrio cholerae. TheT6SS-mediated bacterial killing assays were performed in absence or presence of different protease inhibitors and with different mutant E. coli strains. Expression levels of selected proteins were monitored using SDS-PAGE and immunoblot analyses.
RESULTS: The T6SS-mediated killing of E. coli by V. cholerae was partly blocked when the serine protease inhibitor Pefabloc was present. E. coli lacking the periplasmic protease inhibitor Ecotin showed enhanced susceptibility to killing by V. cholerae. Mutations affecting E. coli membrane stability also caused increased susceptibility to killing by V. cholerae. E. coli lacking the maltodextrin porin protein LamB showed reduced susceptibility to killing by V. cholerae whereas E. coli with induced high levels of LamB showed reduced survival in inter-bacterial competition.
CONCLUSIONS: Our study identified two proteins in E. coli, the intrinsic protease inhibitor Ecotin and the outer membrane porin LamB, that influenced E. coli susceptibility to T6SS-mediated killing by V. cholerae.
GENERAL SIGNIFICANCE: We envision that it is feasible to explore these findings to target and modulate their expression to obtain desired changes in inter-bacterial competition in vivo, e.g. in the gastrointestinal microbiome.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1865 |
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| Enthalten in: |
Biochimica et biophysica acta. General subjects - 1865(2021), 7 vom: 11. Juli, Seite 129912 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Myint, Si Lhyam [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 09.09.2021 Date Revised 09.09.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bbagen.2021.129912 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM324456301 |
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| 100 | 1 | |a Myint, Si Lhyam |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Ecotin and LamB in Escherichia coli influence the susceptibility to Type VI secretion-mediated interbacterial competition and killing by Vibrio cholerae |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 09.09.2021 | ||
| 500 | |a Date Revised 09.09.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a BACKGROUND: A prevailing action of the Type VI secretion system (T6SS) in several Gram-negative bacterial species is inter-bacterial competition. In the past several years, many effectors of T6SS were identified in different bacterial species and their involvement in inter-bacterial interactions were described. However, possible defence mechanisms against T6SS attack among prey bacteria were not well clarified yet | ||
| 520 | |a METHODS: Escherichia coli was assessed for susceptibility to T6SS-mediated killing by Vibrio cholerae. TheT6SS-mediated bacterial killing assays were performed in absence or presence of different protease inhibitors and with different mutant E. coli strains. Expression levels of selected proteins were monitored using SDS-PAGE and immunoblot analyses | ||
| 520 | |a RESULTS: The T6SS-mediated killing of E. coli by V. cholerae was partly blocked when the serine protease inhibitor Pefabloc was present. E. coli lacking the periplasmic protease inhibitor Ecotin showed enhanced susceptibility to killing by V. cholerae. Mutations affecting E. coli membrane stability also caused increased susceptibility to killing by V. cholerae. E. coli lacking the maltodextrin porin protein LamB showed reduced susceptibility to killing by V. cholerae whereas E. coli with induced high levels of LamB showed reduced survival in inter-bacterial competition | ||
| 520 | |a CONCLUSIONS: Our study identified two proteins in E. coli, the intrinsic protease inhibitor Ecotin and the outer membrane porin LamB, that influenced E. coli susceptibility to T6SS-mediated killing by V. cholerae | ||
| 520 | |a GENERAL SIGNIFICANCE: We envision that it is feasible to explore these findings to target and modulate their expression to obtain desired changes in inter-bacterial competition in vivo, e.g. in the gastrointestinal microbiome | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Ecotin | |
| 650 | 4 | |a Escherichia coli | |
| 650 | 4 | |a Interbacterial competition | |
| 650 | 4 | |a LamB | |
| 650 | 4 | |a T6SS | |
| 650 | 4 | |a Vibrio cholerae | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 650 | 7 | |a Bacterial Outer Membrane Proteins |2 NLM | |
| 650 | 7 | |a Eco protein, E coli |2 NLM | |
| 650 | 7 | |a Escherichia coli Proteins |2 NLM | |
| 650 | 7 | |a Periplasmic Proteins |2 NLM | |
| 650 | 7 | |a Porins |2 NLM | |
| 650 | 7 | |a Receptors, Virus |2 NLM | |
| 650 | 7 | |a Type VI Secretion Systems |2 NLM | |
| 650 | 7 | |a maltoporins |2 NLM | |
| 700 | 1 | |a Zlatkov, Nikola |e verfasserin |4 aut | |
| 700 | 1 | |a Aung, Kyaw Min |e verfasserin |4 aut | |
| 700 | 1 | |a Toh, Eric |e verfasserin |4 aut | |
| 700 | 1 | |a Sjöström, Annika |e verfasserin |4 aut | |
| 700 | 1 | |a Nadeem, Aftab |e verfasserin |4 aut | |
| 700 | 1 | |a Duperthuy, Marylise |e verfasserin |4 aut | |
| 700 | 1 | |a Uhlin, Bernt Eric |e verfasserin |4 aut | |
| 700 | 1 | |a Wai, Sun Nyunt |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:1865 |g year:2021 |g number:7 |g day:11 |g month:07 |g pages:129912 |
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