The artificial digestion method underestimates the viability of Anisakis simplex (s.l.) L3 present in processed fish products
© 2021 The Authors. Published by Elsevier Inc. on behalf of International Association of Food and Waterborne Parasitology..
This work studied the performance of the artificial digestion method in terms of recovery and viability of Anisakis simplex third-stage larvae (L3) when previous treatments given to the infected fish muscle may accidentally render viable larvae. For that: a) hake mince was spiked with 10 L3/75g mince, frozen at -10, -15, -20, and -30 °C and immediately thawed, or stored for 12 or 24 h, and subjected to pepsin digestion; b) the mince was spiked under the same conditions, frozen at the above temperatures and thawed immediately. After manual recovery, L3 were assessed for viability, used to spike again the minced fish and subjected to pepsin digestion; c) the mince was spiked with 10 L3 which were: i) living (i.e. chilled), ii) freeze-surviving (live L3 had been previously recovered after freezing at -10 °C), or iii) dead (frozen at -30 °C or - 80 °C), and then subjected to pepsin digestion. Results showed that the artificial digestion method kills a significant number of larvae that may have survived freezing and thus may underestimate the number of viable larvae in a given batch. The method may also underestimate the infection level of fish batches containing dead larvae. It is suggested to take these limitations into account when designing digestion protocols for specific applications, especially when there is a risk of insufficiently treated or cooked fish batches or ready-to-eat foods.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
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| Enthalten in: |
Food and waterborne parasitology - 23(2021) vom: 09. Juni, Seite e00121 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sánchez-Alonso, Isabel [VerfasserIn] |
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| Links: |
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| Themen: |
Anisakis larvae |
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| Anmerkungen: |
Date Revised 07.04.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.fawpar.2021.e00121 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM324058721 |
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| 100 | 1 | |a Sánchez-Alonso, Isabel |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The artificial digestion method underestimates the viability of Anisakis simplex (s.l.) L3 present in processed fish products |
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| 500 | |a Date Revised 07.04.2024 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2021 The Authors. Published by Elsevier Inc. on behalf of International Association of Food and Waterborne Parasitology. | ||
| 520 | |a This work studied the performance of the artificial digestion method in terms of recovery and viability of Anisakis simplex third-stage larvae (L3) when previous treatments given to the infected fish muscle may accidentally render viable larvae. For that: a) hake mince was spiked with 10 L3/75g mince, frozen at -10, -15, -20, and -30 °C and immediately thawed, or stored for 12 or 24 h, and subjected to pepsin digestion; b) the mince was spiked under the same conditions, frozen at the above temperatures and thawed immediately. After manual recovery, L3 were assessed for viability, used to spike again the minced fish and subjected to pepsin digestion; c) the mince was spiked with 10 L3 which were: i) living (i.e. chilled), ii) freeze-surviving (live L3 had been previously recovered after freezing at -10 °C), or iii) dead (frozen at -30 °C or - 80 °C), and then subjected to pepsin digestion. Results showed that the artificial digestion method kills a significant number of larvae that may have survived freezing and thus may underestimate the number of viable larvae in a given batch. The method may also underestimate the infection level of fish batches containing dead larvae. It is suggested to take these limitations into account when designing digestion protocols for specific applications, especially when there is a risk of insufficiently treated or cooked fish batches or ready-to-eat foods | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Anisakis larvae | |
| 650 | 4 | |a Artificial digestion method | |
| 650 | 4 | |a Fish muscle | |
| 650 | 4 | |a Freeze-surviving larvae | |
| 650 | 4 | |a Viability | |
| 700 | 1 | |a Rodríguez, Santiago |e verfasserin |4 aut | |
| 700 | 1 | |a Tejada, Margarita |e verfasserin |4 aut | |
| 700 | 1 | |a Navas, Alfonso |e verfasserin |4 aut | |
| 700 | 1 | |a González-Muñoz, Miguel |e verfasserin |4 aut | |
| 700 | 1 | |a Careche, Mercedes |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:23 |g year:2021 |g day:09 |g month:06 |g pages:e00121 |
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