Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1
Copyright © 2021 Elsevier Ltd. All rights reserved..
Aflatoxin B1 (AFB1) is a mycotoxin often found in food and livestock feed. It can affect human and animal health and is especially damaging to the liver. This study aims to evaluate whether Bacillus amyloliquefaciens (hereafter referred to as B. amyloliquefaciens) B10 can alleviate the toxic effects of AFB1 and, if so, what mechanism is responsible for its action. Specific pathogen-free (SPF) Kunming mice (5-6 weeks old) were divided into four groups (Control, AFB1, B10 strain, and AFB1 + B10 strain) and conducted continuously via gavage for 28 days. Oxidation indices (MDA, T-AOC, SOD, GSH-Px, and CAT) were then measured using their liver tissues and liver coefficient were calculated. Apoptosis was determined using the TUNEL method. Gene expression was determined for Bax, Bcl-2, BIP, CHOP, JNK, Caspase-12, Caspase-9, and Caspase-3, and protein expression was detected for Bax, Bcl-2, and Caspase-3. Our results showed that AFB1 induced the oxidative damage and apoptosis in the livers of mice. However, for mice given B. amyloliquefaciens B10, the biochemical indices, pathological changes, the expressions of genes and proteins related to oxidative stress and apoptosis were significantly reversed. The results indicate that B. amyloliquefaciens B10 antagonizes oxidative damage and apoptosis induced by AFB1 in the livers of mice. The results of this study are of significance for the future use of this strain to reduce the harm of AFB1 to human health and animal reproductive performance.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:151 |
|---|---|
| Enthalten in: |
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association - 151(2021) vom: 15. Mai, Seite 112124 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Li, Xiaotong [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
9N2N2Y55MH |
|---|
| Anmerkungen: |
Date Completed 14.09.2021 Date Revised 14.09.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.fct.2021.112124 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM322838134 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM322838134 | ||
| 003 | DE-627 | ||
| 005 | 20231225182906.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.fct.2021.112124 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1076.xml |
| 035 | |a (DE-627)NLM322838134 | ||
| 035 | |a (NLM)33727180 | ||
| 035 | |a (PII)S0278-6915(21)00157-5 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Li, Xiaotong |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1 |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 14.09.2021 | ||
| 500 | |a Date Revised 14.09.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Aflatoxin B1 (AFB1) is a mycotoxin often found in food and livestock feed. It can affect human and animal health and is especially damaging to the liver. This study aims to evaluate whether Bacillus amyloliquefaciens (hereafter referred to as B. amyloliquefaciens) B10 can alleviate the toxic effects of AFB1 and, if so, what mechanism is responsible for its action. Specific pathogen-free (SPF) Kunming mice (5-6 weeks old) were divided into four groups (Control, AFB1, B10 strain, and AFB1 + B10 strain) and conducted continuously via gavage for 28 days. Oxidation indices (MDA, T-AOC, SOD, GSH-Px, and CAT) were then measured using their liver tissues and liver coefficient were calculated. Apoptosis was determined using the TUNEL method. Gene expression was determined for Bax, Bcl-2, BIP, CHOP, JNK, Caspase-12, Caspase-9, and Caspase-3, and protein expression was detected for Bax, Bcl-2, and Caspase-3. Our results showed that AFB1 induced the oxidative damage and apoptosis in the livers of mice. However, for mice given B. amyloliquefaciens B10, the biochemical indices, pathological changes, the expressions of genes and proteins related to oxidative stress and apoptosis were significantly reversed. The results indicate that B. amyloliquefaciens B10 antagonizes oxidative damage and apoptosis induced by AFB1 in the livers of mice. The results of this study are of significance for the future use of this strain to reduce the harm of AFB1 to human health and animal reproductive performance | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Aflatoxin B1 | |
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a Bacillus amyloliquefaciens B10 | |
| 650 | 4 | |a Liver | |
| 650 | 4 | |a Oxidative stress | |
| 650 | 7 | |a Aflatoxin B1 |2 NLM | |
| 650 | 7 | |a 9N2N2Y55MH |2 NLM | |
| 700 | 1 | |a Lv, Zhiming |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Jia |e verfasserin |4 aut | |
| 700 | 1 | |a Nepovimova, Eugenie |e verfasserin |4 aut | |
| 700 | 1 | |a Long, Miao |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Wenda |e verfasserin |4 aut | |
| 700 | 1 | |a Kuca, Kamil |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association |d 1982 |g 151(2021) vom: 15. Mai, Seite 112124 |w (DE-627)NLM012624691 |x 1873-6351 |7 nnns |
| 773 | 1 | 8 | |g volume:151 |g year:2021 |g day:15 |g month:05 |g pages:112124 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.fct.2021.112124 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 151 |j 2021 |b 15 |c 05 |h 112124 | ||