Effect of fecal microbiota transplantation on neurological restoration in a spinal cord injury mouse model : involvement of brain-gut axis
BACKGROUND: Spinal cord injury (SCI) patients display disruption of gut microbiome, and gut dysbiosis exacerbate neurological impairment in SCI models. Cumulative data support an important role of gut microbiome in SCI. Here, we investigated the hypothesis that fecal microbiota transplantation (FMT) from healthy uninjured mice into SCI mice may exert a neuroprotective effect.
RESULTS: FMT facilitated functional recovery, promoted neuronal axonal regeneration, improved animal weight gain and metabolic profiling, and enhanced intestinal barrier integrity and GI motility in SCI mice. High-throughput sequencing revealed that levels of phylum Firmicutes, family Christensenellaceae, and genus Butyricimonas were reduced in fecal samples of SCI mice, and FMT remarkably reshaped gut microbiome. Also, FMT-treated SCI mice showed increased amount of fecal short-chain fatty acids (SCFAs), which correlated with alteration of intestinal permeability and locomotor recovery. Furthermore, FMT downregulated IL-1β/NF-κB signaling in spinal cord and NF-κB signaling in gut following SCI.
CONCLUSION: Our study demonstrates that reprogramming of gut microbiota by FMT improves locomotor and GI functions in SCI mice, possibly through the anti-inflammatory functions of SCFAs. Video Abstract.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
Microbiome - 9(2021), 1 vom: 07. März, Seite 59 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jing, Yingli [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 16.03.2021 Date Revised 16.03.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1186/s40168-021-01007-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM322354277 |
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| 520 | |a BACKGROUND: Spinal cord injury (SCI) patients display disruption of gut microbiome, and gut dysbiosis exacerbate neurological impairment in SCI models. Cumulative data support an important role of gut microbiome in SCI. Here, we investigated the hypothesis that fecal microbiota transplantation (FMT) from healthy uninjured mice into SCI mice may exert a neuroprotective effect | ||
| 520 | |a RESULTS: FMT facilitated functional recovery, promoted neuronal axonal regeneration, improved animal weight gain and metabolic profiling, and enhanced intestinal barrier integrity and GI motility in SCI mice. High-throughput sequencing revealed that levels of phylum Firmicutes, family Christensenellaceae, and genus Butyricimonas were reduced in fecal samples of SCI mice, and FMT remarkably reshaped gut microbiome. Also, FMT-treated SCI mice showed increased amount of fecal short-chain fatty acids (SCFAs), which correlated with alteration of intestinal permeability and locomotor recovery. Furthermore, FMT downregulated IL-1β/NF-κB signaling in spinal cord and NF-κB signaling in gut following SCI | ||
| 520 | |a CONCLUSION: Our study demonstrates that reprogramming of gut microbiota by FMT improves locomotor and GI functions in SCI mice, possibly through the anti-inflammatory functions of SCFAs. Video Abstract | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Video-Audio Media | |
| 650 | 4 | |a Fecal microbiota transplantation | |
| 650 | 4 | |a GI function | |
| 650 | 4 | |a Gut microbiota | |
| 650 | 4 | |a Neuroinflammation | |
| 650 | 4 | |a Neurological function | |
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| 700 | 1 | |a Bai, Fan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Limiao |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Degang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Chao |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Chuan |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Mingliang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Dong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Yanbing |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jianjun |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Zhiguo |e verfasserin |4 aut | |
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