In Vivo Whole-Cell Patch-Clamp Methods : Recent Technical Progress and Future Perspectives
Brain functions are fundamental for the survival of organisms, and they are supported by neural circuits consisting of a variety of neurons. To investigate the function of neurons at the single-cell level, researchers often use whole-cell patch-clamp recording techniques. These techniques enable us to record membrane potentials (including action potentials) of individual neurons of not only anesthetized but also actively behaving animals. This whole-cell recording method enables us to reveal how neuronal activities support brain function at the single-cell level. In this review, we introduce previous studies using in vivo patch-clamp recording techniques and recent findings primarily regarding neuronal activities in the hippocampus for behavioral function. We further discuss how we can bridge the gap between electrophysiology and biochemistry.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2021 |
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Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
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Enthalten in: |
Sensors (Basel, Switzerland) - 21(2021), 4 vom: 19. Feb. |
Sprache: |
Englisch |
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Beteiligte Personen: |
Noguchi, Asako [VerfasserIn] |
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Links: |
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Themen: |
Axon |
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Date Completed 15.03.2021 Date Revised 10.11.2023 published: Electronic Citation Status MEDLINE |
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doi: |
10.3390/s21041448 |
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funding: |
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PPN (Katalog-ID): |
NLM322269423 |
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520 | |a Brain functions are fundamental for the survival of organisms, and they are supported by neural circuits consisting of a variety of neurons. To investigate the function of neurons at the single-cell level, researchers often use whole-cell patch-clamp recording techniques. These techniques enable us to record membrane potentials (including action potentials) of individual neurons of not only anesthetized but also actively behaving animals. This whole-cell recording method enables us to reveal how neuronal activities support brain function at the single-cell level. In this review, we introduce previous studies using in vivo patch-clamp recording techniques and recent findings primarily regarding neuronal activities in the hippocampus for behavioral function. We further discuss how we can bridge the gap between electrophysiology and biochemistry | ||
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