Binding of the SARS-CoV-2 spike protein to glycans

© 2021 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved..

The pandemic of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a high number of deaths in the world. To combat it, it is necessary to develop a better understanding of how the virus infects host cells. Infection normally starts with the attachment of the virus to cell-surface glycans like heparan sulfate (HS) and sialic acid-containing glycolipids/glycoproteins. In this study, we examined and compared the binding of the subunits and spike (S) proteins of SARS-CoV-2, SARS-CoV, and Middle East respiratory disease (MERS)-CoV to these glycans. Our results revealed that the S proteins and subunits can bind to HS in a sulfation-dependent manner and no binding with sialic acid residues was detected. Overall, this work suggests that HS binding may be a general mechanism for the attachment of these coronaviruses to host cells, and supports the potential importance of HS in infection and in the development of antiviral agents against these viruses.

Media Type:

Electronic Article

Year of Publication:

2021

Contained In:

Science bulletin - Vol. 66, No. 12 (2021), p. 1205-1214

Language:

English

Contributors:

Hao, Wei
Ma, Bo
Li, Ziheng
Wang, Xiaoyu
Gao, Xiaopan
Li, Yaohao
Qin, Bo
Shang, Shiying
Cui, Sheng
Tan, Zhongping

Links:

Volltext

Keywords:

Glycan microarray
Heparan sulfate
Journal Article
SARS-CoV-2 S protein
Sialic acid
Sulfation
Surface plasmon resonance

Notes:

Date Revised 03.06.2021

published: Print-Electronic

Citation Status PubMed-not-MEDLINE

Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Physical Description:

Online-Ressource

doi:

10.1016/j.scib.2021.01.010

PMID:

33495714

PPN (Catalogue-ID):

NLM321612361