Detection and characterization of rotavirus G and P types from children with acute gastroenteritis in Qom, central Iran
©2020 RIGLD, Research Institute for Gastroenterology and Liver Diseases..
AIM: The aim of the study is to estimate the burden of Rotavirus gastroenteritis as well as predominant genotypes of Rotavirus among children less than 5 years of age referring to Pediatric University Hospital in Qom, Iran.
BACKGROUND: Gastroenteritis is the fourth most common cause of death and accounts for 16% of all deaths in children <5 years of age worldwide.
METHODS: During two years, 130 patients referring to a pediatric hospital were enrolled in this study. After RNA extraction, Rotaviruses were detected by the VP6 gene. Then, G-typing (G1, G2, G3, G4, G8, G9, and G12) and P-typing (P4, P6, and P8) were performed using RT-PCR and specific primers.
RESULTS: The results of the PCR revealed that from a total of 130 patients, 22 cases (16.9%) showed positive VP6 by RT-PCR. G1 was mostly the predominant serotype (27%), accounting for 22% of all VP7-positive isolates, followed by G9 (18%), G2 (9%), G3 (9%), and G4 (9%). None of the strains revealed the presence of G8 genotype (0%), and 5 specimens (23%) were non-typable. The frequency of P typing was P8 (50%), P6 (23%), P4 (14%), and 3 samples were P-non-typable (13%), respectively. The dominant G-P combination was G1 [8] (32%).
CONCLUSION: Such studies based on typing methods assists in the Rotavirus vaccine introduction by policymakers and design of new effective vaccines.
Medienart: |
Artikel |
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Erscheinungsjahr: |
2020 |
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Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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Enthalten in: |
Gastroenterology and hepatology from bed to bench - 13(2020), Suppl1 vom: 27., Seite S128-S133 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Shams, Saeed [VerfasserIn] |
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Themen: |
Acute gastroenteritis |
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Anmerkungen: |
Date Revised 10.11.2023 published: Print Citation Status PubMed-not-MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM321446461 |
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500 | |a published: Print | ||
500 | |a Citation Status PubMed-not-MEDLINE | ||
520 | |a ©2020 RIGLD, Research Institute for Gastroenterology and Liver Diseases. | ||
520 | |a AIM: The aim of the study is to estimate the burden of Rotavirus gastroenteritis as well as predominant genotypes of Rotavirus among children less than 5 years of age referring to Pediatric University Hospital in Qom, Iran | ||
520 | |a BACKGROUND: Gastroenteritis is the fourth most common cause of death and accounts for 16% of all deaths in children <5 years of age worldwide | ||
520 | |a METHODS: During two years, 130 patients referring to a pediatric hospital were enrolled in this study. After RNA extraction, Rotaviruses were detected by the VP6 gene. Then, G-typing (G1, G2, G3, G4, G8, G9, and G12) and P-typing (P4, P6, and P8) were performed using RT-PCR and specific primers | ||
520 | |a RESULTS: The results of the PCR revealed that from a total of 130 patients, 22 cases (16.9%) showed positive VP6 by RT-PCR. G1 was mostly the predominant serotype (27%), accounting for 22% of all VP7-positive isolates, followed by G9 (18%), G2 (9%), G3 (9%), and G4 (9%). None of the strains revealed the presence of G8 genotype (0%), and 5 specimens (23%) were non-typable. The frequency of P typing was P8 (50%), P6 (23%), P4 (14%), and 3 samples were P-non-typable (13%), respectively. The dominant G-P combination was G1 [8] (32%) | ||
520 | |a CONCLUSION: Such studies based on typing methods assists in the Rotavirus vaccine introduction by policymakers and design of new effective vaccines | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Diarrhea | |
650 | 4 | |a Genotyping | |
650 | 4 | |a Iran | |
650 | 4 | |a Rotavirus | |
650 | 4 | |a acute gastroenteritis | |
650 | 4 | |a vaccine | |
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