Detection and characterization of rotavirus G and P types from children with acute gastroenteritis in Qom, central Iran
©2020 RIGLD, Research Institute for Gastroenterology and Liver Diseases..
AIM: The aim of the study is to estimate the burden of Rotavirus gastroenteritis as well as predominant genotypes of Rotavirus among children less than 5 years of age referring to Pediatric University Hospital in Qom, Iran.
BACKGROUND: Gastroenteritis is the fourth most common cause of death and accounts for 16% of all deaths in children <5 years of age worldwide.
METHODS: During two years, 130 patients referring to a pediatric hospital were enrolled in this study. After RNA extraction, Rotaviruses were detected by the VP6 gene. Then, G-typing (G1, G2, G3, G4, G8, G9, and G12) and P-typing (P4, P6, and P8) were performed using RT-PCR and specific primers.
RESULTS: The results of the PCR revealed that from a total of 130 patients, 22 cases (16.9%) showed positive VP6 by RT-PCR. G1 was mostly the predominant serotype (27%), accounting for 22% of all VP7-positive isolates, followed by G9 (18%), G2 (9%), G3 (9%), and G4 (9%). None of the strains revealed the presence of G8 genotype (0%), and 5 specimens (23%) were non-typable. The frequency of P typing was P8 (50%), P6 (23%), P4 (14%), and 3 samples were P-non-typable (13%), respectively. The dominant G-P combination was G1 [8] (32%).
CONCLUSION: Such studies based on typing methods assists in the Rotavirus vaccine introduction by policymakers and design of new effective vaccines.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
|---|---|
| Enthalten in: |
Gastroenterology and hepatology from bed to bench - 13(2020), Suppl1 vom: 27., Seite S128-S133 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Shams, Saeed [VerfasserIn] |
|---|
| Themen: |
Acute gastroenteritis |
|---|
| Anmerkungen: |
Date Revised 10.11.2023 published: Print Citation Status PubMed-not-MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM321446461 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM321446461 | ||
| 003 | DE-627 | ||
| 005 | 20231225175826.0 | ||
| 007 | tu | ||
| 008 | 231225s2020 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n1071.xml |
| 035 | |a (DE-627)NLM321446461 | ||
| 035 | |a (NLM)33585014 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Shams, Saeed |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detection and characterization of rotavirus G and P types from children with acute gastroenteritis in Qom, central Iran |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Revised 10.11.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a ©2020 RIGLD, Research Institute for Gastroenterology and Liver Diseases. | ||
| 520 | |a AIM: The aim of the study is to estimate the burden of Rotavirus gastroenteritis as well as predominant genotypes of Rotavirus among children less than 5 years of age referring to Pediatric University Hospital in Qom, Iran | ||
| 520 | |a BACKGROUND: Gastroenteritis is the fourth most common cause of death and accounts for 16% of all deaths in children <5 years of age worldwide | ||
| 520 | |a METHODS: During two years, 130 patients referring to a pediatric hospital were enrolled in this study. After RNA extraction, Rotaviruses were detected by the VP6 gene. Then, G-typing (G1, G2, G3, G4, G8, G9, and G12) and P-typing (P4, P6, and P8) were performed using RT-PCR and specific primers | ||
| 520 | |a RESULTS: The results of the PCR revealed that from a total of 130 patients, 22 cases (16.9%) showed positive VP6 by RT-PCR. G1 was mostly the predominant serotype (27%), accounting for 22% of all VP7-positive isolates, followed by G9 (18%), G2 (9%), G3 (9%), and G4 (9%). None of the strains revealed the presence of G8 genotype (0%), and 5 specimens (23%) were non-typable. The frequency of P typing was P8 (50%), P6 (23%), P4 (14%), and 3 samples were P-non-typable (13%), respectively. The dominant G-P combination was G1 [8] (32%) | ||
| 520 | |a CONCLUSION: Such studies based on typing methods assists in the Rotavirus vaccine introduction by policymakers and design of new effective vaccines | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Diarrhea | |
| 650 | 4 | |a Genotyping | |
| 650 | 4 | |a Iran | |
| 650 | 4 | |a Rotavirus | |
| 650 | 4 | |a acute gastroenteritis | |
| 650 | 4 | |a vaccine | |
| 700 | 1 | |a Mousavi Nasab, Seyed Dawood |e verfasserin |4 aut | |
| 700 | 1 | |a Heydari, Hosein |e verfasserin |4 aut | |
| 700 | 1 | |a Tafaroji, Javad |e verfasserin |4 aut | |
| 700 | 1 | |a Ahmadi, Nayebali |e verfasserin |4 aut | |
| 700 | 1 | |a Shams Afzali, Esmaeil |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Gastroenterology and hepatology from bed to bench |d 2011 |g 13(2020), Suppl1 vom: 27., Seite S128-S133 |w (DE-627)NLM238305023 |x 2008-2258 |7 nnns |
| 773 | 1 | 8 | |g volume:13 |g year:2020 |g number:Suppl1 |g day:27 |g pages:S128-S133 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 13 |j 2020 |e Suppl1 |b 27 |h S128-S133 | ||