Changes in mRNA and protein levels of gonadotropin releasing hormone and receptor in ovine thymus, lymph node, spleen, and liver during early pregnancy

Copyright © 2021 Elsevier Inc. All rights reserved..

There exists maternal immunological modification in maternal immune organs during early pregnancy in mammals. Gonadotropin releasing hormone (GnRH) is widely distributed in vertebrate tissues, including immune organs. However, it is unclear that early pregnancy induces expression of GnRH and GnRH receptor (GnRHR) in ovine immune organs. The objective of this study was to explore the expression of GnRH and GnRHR in main immune organs (thymus, lymph node, spleen, and liver) during early pregnancy in sheep. Ovine thymus, lymph node, spleen and liver were sampled at day 16 of estrous cycle, and days 13, 16, and 25 of pregnancy. The expression of GnRH and GnRHR was detected through real-time quantitative PCR, Western blot and immunohistochemistry analysis. The results indicated that early pregnancy induced upregulation of mRNA and protein levels of GnRH and GnRHR in the maternal lymph node, spleen and liver, and mRNA and protein of GnRH in the maternal thymus, but mRNA and protein of GnRHR decreased in the maternal thymus during early pregnancy. In summary, the mRNA and protein levels of GnRH and GnRHR were changed in maternal thymus, lymph node, spleen and liver in a tissue specific manner during early pregnancy in sheep.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:76

Enthalten in:

Domestic animal endocrinology - 76(2021) vom: 15. Juli, Seite 106607

Sprache:

Englisch

Beteiligte Personen:

Cao, N [VerfasserIn]
Cao, L [VerfasserIn]
Gao, M [VerfasserIn]
Wang, H [VerfasserIn]
Zhang, L [VerfasserIn]
Yang, L [VerfasserIn]

Links:

Volltext

Themen:

33515-09-2
Gonadotropin releasing hormone
Gonadotropin-Releasing Hormone
Journal Article
Liver
Lymph node
RNA, Messenger
Research Support, Non-U.S. Gov't
Spleen
Thymus

Anmerkungen:

Date Completed 31.03.2022

Date Revised 01.04.2022

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.domaniend.2021.106607

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM321420608