FISH in Suspension or in Adherent Cells
Fluorescence in situ hybridization (FISH) enables the detection and enumeration of microorganisms in a diversity of samples. Short-length oligonucleotide DNA probes complementary to 16S or 23S rRNA sequences are generally used to target different phylogenetic levels. The protocol for the application of FISH to aggregated or suspended cells in mixed microbial communities is described in this chapter, with a special emphasis on environmental samples.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2246 |
|---|---|
| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2246(2021) vom: 12., Seite 51-67 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Di Pippo, Francesca [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 30.03.2021 Date Revised 30.03.2021 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1007/978-1-0716-1115-9_4 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM321368487 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM321368487 | ||
| 003 | DE-627 | ||
| 005 | 20231225175647.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/978-1-0716-1115-9_4 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1071.xml |
| 035 | |a (DE-627)NLM321368487 | ||
| 035 | |a (NLM)33576982 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Di Pippo, Francesca |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a FISH in Suspension or in Adherent Cells |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 30.03.2021 | ||
| 500 | |a Date Revised 30.03.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Fluorescence in situ hybridization (FISH) enables the detection and enumeration of microorganisms in a diversity of samples. Short-length oligonucleotide DNA probes complementary to 16S or 23S rRNA sequences are generally used to target different phylogenetic levels. The protocol for the application of FISH to aggregated or suspended cells in mixed microbial communities is described in this chapter, with a special emphasis on environmental samples | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Adherent cells | |
| 650 | 4 | |a Environmental samples | |
| 650 | 4 | |a FISH | |
| 650 | 4 | |a Oligonucleotide probes | |
| 650 | 4 | |a Suspension cells | |
| 650 | 4 | |a Unculturable bacteria | |
| 650 | 7 | |a Oligonucleotide Probes |2 NLM | |
| 650 | 7 | |a RNA, Ribosomal, 16S |2 NLM | |
| 650 | 7 | |a RNA, Ribosomal, 23S |2 NLM | |
| 700 | 1 | |a Queirós, Diogo |e verfasserin |4 aut | |
| 700 | 1 | |a Pereira, Joana |e verfasserin |4 aut | |
| 700 | 1 | |a Lemos, Paulo C |e verfasserin |4 aut | |
| 700 | 1 | |a Serafim, Luísa S |e verfasserin |4 aut | |
| 700 | 1 | |a Rossetti, Simona |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Methods in molecular biology (Clifton, N.J.) |d 1984 |g 2246(2021) vom: 12., Seite 51-67 |w (DE-627)NLM074849794 |x 1940-6029 |7 nnns |
| 773 | 1 | 8 | |g volume:2246 |g year:2021 |g day:12 |g pages:51-67 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/978-1-0716-1115-9_4 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 2246 |j 2021 |b 12 |h 51-67 | ||