Effective Separation for New Therapeutic Modalities Utilizing Temperature-responsive Chromatography
In recent years, drug discovery and therapeutics trends have shifted from a focus on small-molecule compounds to biopharmaceuticals, genes, cell therapy, and regenerative medicine. Therefore, new approaches and technologies must be developed to respond to these changes in medical care. To achieve this, we applied a temperature-responsive separation system to purify a variety of proteins and cells. We developed a temperature-responsive chromatography technique based on a poly(N-isopropylacrylamide) (PNIPAAm)-grafted stationary phase. This method may be applied to various types of protein and cell separation applications by optimizing the properties of the modified polymers used in this system. Therefore, the developed temperature-responsive HPLC columns and temperature-responsive solid-phase extraction (TR-SPE) columns can be an effective separation tool for new therapeutic modalities such as monoclonal antibodies, nucleic acid drugs, and cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
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| Enthalten in: |
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 37(2021), 5 vom: 10. Mai, Seite 651-660 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Maekawa, Yutaro [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 25.10.2021 Date Revised 08.08.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.2116/analsci.20SCR09 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM320797902 |
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| 520 | |a In recent years, drug discovery and therapeutics trends have shifted from a focus on small-molecule compounds to biopharmaceuticals, genes, cell therapy, and regenerative medicine. Therefore, new approaches and technologies must be developed to respond to these changes in medical care. To achieve this, we applied a temperature-responsive separation system to purify a variety of proteins and cells. We developed a temperature-responsive chromatography technique based on a poly(N-isopropylacrylamide) (PNIPAAm)-grafted stationary phase. This method may be applied to various types of protein and cell separation applications by optimizing the properties of the modified polymers used in this system. Therefore, the developed temperature-responsive HPLC columns and temperature-responsive solid-phase extraction (TR-SPE) columns can be an effective separation tool for new therapeutic modalities such as monoclonal antibodies, nucleic acid drugs, and cells | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Nagase, Kenichi |e verfasserin |4 aut | |
| 700 | 1 | |a Kanazawa, Hideko |e verfasserin |4 aut | |
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