Plant Growth Promotion at Low Temperature by Phosphate-Solubilizing Pseudomonas Spp. Isolated from High-Altitude Himalayan Soil

© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature..

Scarcity of arable land, limited soil nutrient availability, and low-temperature conditions in the Himalayan regions need to be smartly managed using sustainable approaches for better crop yields. Microorganisms, able to efficiently solubilize phosphate at low temperatures, provide an opportunity to promote plant growth in an ecofriendly way. In this study, we have investigated the ability of psychrotolerant Pseudomonas spp., isolated from high altitudes of Indian Himalaya to solubilize P at low temperature. Quantitative estimation of phosphate solubilization and production of relevant enzymes at two different temperatures (15 and 25 °C) was performed for 4 out of 11 selected isolates, namely, GBPI_506 (Pseudomonas sp.), GBPI_508 (Pseudomonas palleroniana), GBPI_Hb61 (Pseudomonas proteolytica), and GBPI_CDB143 (Pseudomonas azotoformans). Among all, isolate GBPI_CDB143 showed highest efficiency to solubilize tri-calcium phosphate (110.50 ± 3.44 μg/mL) at 25 °C after 6 days while the culture supernatants of isolate GBPI_506 displayed the highest phytase activity (15.91 ± 0.35 U/mL) at 15 °C and alkaline phosphatase (3.09 ± 0.07 U/mL) at 25 °C in 6 and 9 days, respectively. Out of five different organic acids quantified, oxalic acid and malic acid were produced in maximum quantity by all four isolates. With the exception of GBPI_508, inoculation of bacteria promoted overall growth (rosette diameter, leaf area, and biomass) of Arabidopsis thaliana plants as compared to uninoculated control plants in growth chamber conditions. The plant growth promotion by each bacterial isolate was further validated by monitoring root colonization in the inoculated plants. These bacterial isolates with low-temperature phosphate solubilization potential along with phosphatases and phytase activity at low temperature could be harnessed for sustainable crop production in P-deficient agricultural soils under mountain ecosystems.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:82

Enthalten in:

Microbial ecology - 82(2021), 3 vom: 05. Okt., Seite 677-687

Sprache:

Englisch

Beteiligte Personen:

Adhikari, Priyanka [VerfasserIn]
Jain, Rahul [VerfasserIn]
Sharma, Avinash [VerfasserIn]
Pandey, Anita [VerfasserIn]

Links:

Volltext

Themen:

Arabidopsis thaliana
Fluorescence microscopy
Indian Himalayan region
Journal Article
Low temperature
Phosphates
Plant growth promotion
Soil

Anmerkungen:

Date Completed 24.11.2021

Date Revised 24.11.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1007/s00248-021-01702-1

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM320740331