Hypolipidemic properties of Chlorella pyrenoidosa organic acids via AMPK/HMGCR/SREBP-1c pathway in vivo
© 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC..
The aim of this study was to explore the effects and mechanisms of 95% ethanol extract of Chlorella pyrenoidosa (CPE95) on lipid metabolism in hyperlipidemic rats. For the sake of chemical composition analysis of CPE95, liquid chromatography-mass spectrometry (LC-MS) was used for determination. After treatment with CPE95, serum high-density lipoprotein cholesterol content of the hyperlipidemic rats was increased, while the contents of cholesterol, triglyceride, and low-density lipoprotein cholesterol were decreased strikingly. Moreover, the result of histopathology analysis showed that the accumulation and fatty deformation of the livers were relieved. Real-time quantitative PCR and Western blotting were used to determine the expression levels of lipid metabolism-related genes. The gene expression level of adenosine 5'-monophosphate-activated protein kinase was descended, and expressions of sterol regulatory element-binding protein-1c, acetyl-CoA carboxylase, and 3-hydroxy-3-methylglutaryl coenzyme A reductase were all downregulated in the CPE95-treated rats. It suggested that CPE95 may effectively improve the hyperlipidemia in rats and would be potential for functional food component to reduce blood lipid.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2021 |
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Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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Enthalten in: |
Food science & nutrition - 9(2021), 1 vom: 22. Jan., Seite 459-468 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Chen, Jie [VerfasserIn] |
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Links: |
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Themen: |
AMPK signaling pathway |
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Anmerkungen: |
Date Revised 22.01.2021 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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doi: |
10.1002/fsn3.2014 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM320356574 |
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520 | |a The aim of this study was to explore the effects and mechanisms of 95% ethanol extract of Chlorella pyrenoidosa (CPE95) on lipid metabolism in hyperlipidemic rats. For the sake of chemical composition analysis of CPE95, liquid chromatography-mass spectrometry (LC-MS) was used for determination. After treatment with CPE95, serum high-density lipoprotein cholesterol content of the hyperlipidemic rats was increased, while the contents of cholesterol, triglyceride, and low-density lipoprotein cholesterol were decreased strikingly. Moreover, the result of histopathology analysis showed that the accumulation and fatty deformation of the livers were relieved. Real-time quantitative PCR and Western blotting were used to determine the expression levels of lipid metabolism-related genes. The gene expression level of adenosine 5'-monophosphate-activated protein kinase was descended, and expressions of sterol regulatory element-binding protein-1c, acetyl-CoA carboxylase, and 3-hydroxy-3-methylglutaryl coenzyme A reductase were all downregulated in the CPE95-treated rats. It suggested that CPE95 may effectively improve the hyperlipidemia in rats and would be potential for functional food component to reduce blood lipid | ||
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700 | 1 | |a Wan, Xuzhi |e verfasserin |4 aut | |
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700 | 1 | |a Wang, Change |e verfasserin |4 aut | |
700 | 1 | |a Zeng, Feng |e verfasserin |4 aut | |
700 | 1 | |a Zhao, Chao |e verfasserin |4 aut | |
700 | 1 | |a Liu, Bin |e verfasserin |4 aut | |
700 | 1 | |a Huang, Ying |e verfasserin |4 aut | |
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