Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved..
In mammals, hearing loss is irreversible due to the lack of regenerative potential of non-sensory cochlear cells. Neonatal cochlear cells, however, can grow into organoids that harbor sensory epithelial cells, including hair cells and supporting cells. Here, we purify different cochlear cell types from neonatal mice, validate the composition of the different groups with single-cell RNA sequencing (RNA-seq), and assess the various groups' potential to grow into inner ear organoids. We find that the greater epithelial ridge (GER), a transient cell population that disappears during post-natal cochlear maturation, harbors the most potent organoid-forming cells. We identified three distinct GER cell groups that correlate with a specific spatial distribution of marker genes. Organoid formation was synergistically enhanced when the cells were cultured at increasing density. This effect is not due to diffusible signals but requires direct cell-to-cell contact. Our findings improve the development of cell-based assays to study culture-generated inner ear cell types.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
|---|---|
| Enthalten in: |
Cell reports - 34(2021), 3 vom: 19. Jan., Seite 108646 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Kubota, Marie [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Auditory periphery |
|---|
| Anmerkungen: |
Date Completed 28.01.2022 Date Revised 30.03.2024 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.celrep.2020.108646 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM320344339 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM320344339 | ||
| 003 | DE-627 | ||
| 005 | 20240330235536.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.celrep.2020.108646 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1357.xml |
| 035 | |a (DE-627)NLM320344339 | ||
| 035 | |a (NLM)33472062 | ||
| 035 | |a (PII)S2211-1247(20)31635-1 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kubota, Marie |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 28.01.2022 | ||
| 500 | |a Date Revised 30.03.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a In mammals, hearing loss is irreversible due to the lack of regenerative potential of non-sensory cochlear cells. Neonatal cochlear cells, however, can grow into organoids that harbor sensory epithelial cells, including hair cells and supporting cells. Here, we purify different cochlear cell types from neonatal mice, validate the composition of the different groups with single-cell RNA sequencing (RNA-seq), and assess the various groups' potential to grow into inner ear organoids. We find that the greater epithelial ridge (GER), a transient cell population that disappears during post-natal cochlear maturation, harbors the most potent organoid-forming cells. We identified three distinct GER cell groups that correlate with a specific spatial distribution of marker genes. Organoid formation was synergistically enhanced when the cells were cultured at increasing density. This effect is not due to diffusible signals but requires direct cell-to-cell contact. Our findings improve the development of cell-based assays to study culture-generated inner ear cell types | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a auditory periphery | |
| 650 | 4 | |a epigenetic reprogramming | |
| 650 | 4 | |a hair cell regeneration | |
| 650 | 4 | |a inner ear organoid | |
| 650 | 4 | |a otic | |
| 650 | 4 | |a supporting cell | |
| 700 | 1 | |a Scheibinger, Mirko |e verfasserin |4 aut | |
| 700 | 1 | |a Jan, Taha A |e verfasserin |4 aut | |
| 700 | 1 | |a Heller, Stefan |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell reports |d 2012 |g 34(2021), 3 vom: 19. Jan., Seite 108646 |w (DE-627)NLM217067492 |x 2211-1247 |7 nnns |
| 773 | 1 | 8 | |g volume:34 |g year:2021 |g number:3 |g day:19 |g month:01 |g pages:108646 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.celrep.2020.108646 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 34 |j 2021 |e 3 |b 19 |c 01 |h 108646 | ||