A "hot Spot"-Enhanced paper lateral flow assay for ultrasensitive detection of traumatic brain injury biomarker S-100β in blood plasma
Copyright © 2021 Elsevier B.V. All rights reserved..
Currently colorimetric paper lateral flow strips (PLFS) encounter two major limitations, that is, low sensitivity and severe interference from complex sample matrices such as blood. These shortcomings limit their application in detection of low-concentration analytes in complex samples. To solve these problems, a PLFS has been developed by utilizing surface-enhanced Raman scattering (SERS) for sensing signal transduction. In particular, a hierarchical three-dimensional nanostructure has been designed to create "hot spots", which can significantly amplify the SERS sensing signal, leading to high sensitivity. As a result, this PLFS has demonstrated a limit of detection (LOD) of 5.0 pg mL-1 toward detection of S-100β, a traumatic brain injury (TBI) protein biomarker in blood plasma. The PLFS has been successfully used for rapid measurement of S-100β in clinical TBI patient samples taken in the emergency department. Availability of PLFS for blood testing would shift the paradigm of TBI patient management and clinical outcome in emergency departments. It is expected that this type of PLFS can be adapted for rapid detection of various human diseases due to its capability of measuring a low level of protein blood biomarkers in complex human fluids.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2021 |
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Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:177 |
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Enthalten in: |
Biosensors & bioelectronics - 177(2021) vom: 01. Apr., Seite 112967 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Gao, Xuefei [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 14.05.2021 Date Revised 16.07.2022 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.bios.2021.112967 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM319926621 |
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520 | |a Copyright © 2021 Elsevier B.V. All rights reserved. | ||
520 | |a Currently colorimetric paper lateral flow strips (PLFS) encounter two major limitations, that is, low sensitivity and severe interference from complex sample matrices such as blood. These shortcomings limit their application in detection of low-concentration analytes in complex samples. To solve these problems, a PLFS has been developed by utilizing surface-enhanced Raman scattering (SERS) for sensing signal transduction. In particular, a hierarchical three-dimensional nanostructure has been designed to create "hot spots", which can significantly amplify the SERS sensing signal, leading to high sensitivity. As a result, this PLFS has demonstrated a limit of detection (LOD) of 5.0 pg mL-1 toward detection of S-100β, a traumatic brain injury (TBI) protein biomarker in blood plasma. The PLFS has been successfully used for rapid measurement of S-100β in clinical TBI patient samples taken in the emergency department. Availability of PLFS for blood testing would shift the paradigm of TBI patient management and clinical outcome in emergency departments. It is expected that this type of PLFS can be adapted for rapid detection of various human diseases due to its capability of measuring a low level of protein blood biomarkers in complex human fluids | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Biomarker | |
650 | 4 | |a Blood testing | |
650 | 4 | |a Paper lateral flow assay | |
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650 | 4 | |a Traumatic brain injury | |
650 | 7 | |a Biomarkers |2 NLM | |
650 | 7 | |a S100 Calcium Binding Protein beta Subunit |2 NLM | |
650 | 7 | |a S100B protein, human |2 NLM | |
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700 | 1 | |a Zheng, Peng |e verfasserin |4 aut | |
700 | 1 | |a Kasani, Sujan |e verfasserin |4 aut | |
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700 | 1 | |a Engler-Chiurazzi, Elizabeth B |e verfasserin |4 aut | |
700 | 1 | |a Simpkins, James W |e verfasserin |4 aut | |
700 | 1 | |a Wigginton, Jane G |e verfasserin |4 aut | |
700 | 1 | |a Wu, Nianqiang |e verfasserin |4 aut | |
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