Extracorporeal high-pressure therapy (EHPT) for malignant melanoma consisting of simultaneous tumor eradication and autologous dermal substitute preparation

© 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V..

Surgical resection of skin tumors leads to large defects in surrounding normal tissues, which should be reconstructed thereafter using the patient's own tissues taken from the other site. Our challenge is to solve this problem in dermal malignant melanoma (MM) by a novel process, named extracorporeal high pressure therapy (EHPT), in which the tissue containing tumor is resected and pressurized, and the treated tissue is re-transplant back to the same position as a tumor-free autologous dermal substitute. The key points are complete tumor death and preservation of native extra cellular matrix (ECM) by the hydrostatic pressure. We found that high hydrostatic pressure at 200 MPa for 10 min at room temperature is completely cytocidal against MM cells in suspension form, in monolayer form, and even in the solid tumor form. MM tumor-bearing nude mice were established by injected human MM cells intradermally and treated by EHTP. The denaturation of the dermal extra cellular matrices was so mild that the pressurized skin was well engrafted as tumor free autologous dermal tissues, resulting in the complete eradication of the MM without any unnecessary skin reconstruction surgery. This very simple and short pressing treatment was proved to make the tumor tissue to the transplantable and tumor-free autologous dermal substitute, which can be applicable to the other temporally resectable tissues.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:15

Enthalten in:

Regenerative therapy - 15(2020) vom: 12. Dez., Seite 187-194

Sprache:

Englisch

Beteiligte Personen:

Jinno, C [VerfasserIn]
Morimoto, N [VerfasserIn]
Mahara, A [VerfasserIn]
Sakamoto, M [VerfasserIn]
Ogino, S [VerfasserIn]
Fujisato, T [VerfasserIn]
Suzuki, S [VerfasserIn]
Yamaoka, T [VerfasserIn]

Links:

Volltext

Themen:

Dermal substitute
Extracorporeal treatment
High hydrostatic pressure
Journal Article
Malignant melanoma

Anmerkungen:

Date Revised 12.01.2021

published: Electronic-eCollection

Citation Status PubMed-not-MEDLINE

doi:

10.1016/j.reth.2020.09.003

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM31989729X