iBRAB : In silico based-designed broad-spectrum Fab against H1N1 influenza A virus

Influenza virus A is a significant agent involved in the outbreak of worldwide epidemics, causing millions of fatalities around the world by respiratory diseases and seasonal illness. Many projects had been conducting to investigate recovered infected patients for therapeutic vaccines that have broad-spectrum activity. With the aid of the computational approach in biology, the designation for a vaccine model is more accessible. We developed an in silico protocol called iBRAB to design a broad-reactive Fab on a wide range of influenza A virus. The Fab model was constructed based on sequences and structures of available broad-spectrum Abs or Fabs against a wide range of H1N1 influenza A virus. As a result, the proposed Fab model followed iBRAB has good binding affinity over 27 selected HA of different strains of H1 influenza A virus, including wild-type and mutated ones. The examination also took by computational tools to fasten the procedure. This protocol could be applied for a fast-designed therapeutic vaccine against different types of threats.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:15

Enthalten in:

PloS one - 15(2020), 12 vom: 01., Seite e0239112

Sprache:

Englisch

Beteiligte Personen:

Do, Phuc-Chau [VerfasserIn]
Nguyen, Trung H [VerfasserIn]
Vo, Uyen H M [VerfasserIn]
Le, Ly [VerfasserIn]

Links:

Volltext

Themen:

Antibodies, Viral
Antigens, Viral
Hemagglutinin Glycoproteins, Influenza Virus
Immunoglobulin Fab Fragments
Influenza Vaccines
Journal Article
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 12.01.2021

Date Revised 10.11.2023

published: Electronic-eCollection

Citation Status MEDLINE

doi:

10.1371/journal.pone.0239112

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM319467651