Smoking effect on the ultrastructural properties of cultured lung myofibroblasts

This study aimed at an ultrastructural characterization of myofibroblasts cultured from different compartments of lung from never-smokers and smokers with or without COPD. In addition, we evaluated the expression of alpha smooth muscle actin (α-SMA), a marker for myofibroblasts, and contractile properties. Stromal cells cultured from central and corresponding peripheral or only from peripheral lung of never-smokers, smokers without COPD and COPD patients were analyzed by transmission electron microscopy (TEM), immunoelectron microscopy (IEM), Western analysis and/or by collagen gel contraction assay. TEM revealed that myofibroblasts cultured from smokers and COPD had less prominent intracellular actin filaments. We also examined fibronexus (FNX), which is a typical ultrastructural feature of myofibroblasts, and observed that patients with COPD more frequently had tandem-like FNX as compared to other samples. Western analysis showed that the samples derived from the central lung of never-smokers expressed higher levels of α-SMA than those of smokers and COPD patients. Cells from central lung were less contractile than those from peripheral lung. We conclude that myofibroblasts have variable ultrastructural and functional properties based on their localization in the lung and, moreover, these properties are affected by both smoking history and COPD.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:45

Enthalten in:

Ultrastructural pathology - 45(2021), 1 vom: 02. Jan., Seite 37-48

Sprache:

Englisch

Beteiligte Personen:

Lehtonen, Siri [VerfasserIn]
Nurmos, Ninni-Ingrid [VerfasserIn]
Karvonen, Henna M [VerfasserIn]
Lappi-Blanco, Elisa [VerfasserIn]
Harju, Terttu [VerfasserIn]
Sköld, Magnus [VerfasserIn]
Kaarteenaho, Riitta [VerfasserIn]

Links:

Volltext

Themen:

Actin
COPD
Contraction
Fibronexus
Journal Article

Anmerkungen:

Date Completed 28.10.2021

Date Revised 28.10.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1080/01913123.2020.1858214

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM319419487