Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles
© 2020 The Author(s)..
Positive-strand RNA viruses replicate in distinct membranous structures called replication organelles (ROs). Mechanistic studies of RO formation have been difficult because perturbations affecting viral replication have an impact on viral protein amounts, thus affecting RO biogenesis. Here, we present a detailed guide on how to use a replication-independent expression system, designated pIRO (plasmid-induced replication organelle formation), inducing bona fide flavivirus ROs in transfected cells. This will be useful for mechanistic studies of viral and cellular factors driving flavivirus RO biogenesis. For complete details on the use and execution of this protocol, please refer to Cerikan et al. (2020).
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2020 |
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Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:1 |
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Enthalten in: |
STAR protocols - 1(2020), 3 vom: 18. Dez., Seite 100173 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Goellner, Sarah [VerfasserIn] |
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Links: |
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Themen: |
Cell Biology |
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Anmerkungen: |
Date Completed 09.03.2022 Date Revised 09.03.2022 published: Electronic-eCollection Citation Status MEDLINE |
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doi: |
10.1016/j.xpro.2020.100173 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM319412032 |
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520 | |a Positive-strand RNA viruses replicate in distinct membranous structures called replication organelles (ROs). Mechanistic studies of RO formation have been difficult because perturbations affecting viral replication have an impact on viral protein amounts, thus affecting RO biogenesis. Here, we present a detailed guide on how to use a replication-independent expression system, designated pIRO (plasmid-induced replication organelle formation), inducing bona fide flavivirus ROs in transfected cells. This will be useful for mechanistic studies of viral and cellular factors driving flavivirus RO biogenesis. For complete details on the use and execution of this protocol, please refer to Cerikan et al. (2020) | ||
650 | 4 | |a Journal Article | |
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700 | 1 | |a Cortese, Mirko |e verfasserin |4 aut | |
700 | 1 | |a Neufeldt, Christopher J |e verfasserin |4 aut | |
700 | 1 | |a Haselmann, Uta |e verfasserin |4 aut | |
700 | 1 | |a Bartenschlager, Ralf |e verfasserin |4 aut | |
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