Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles

© 2020 The Author(s)..

Positive-strand RNA viruses replicate in distinct membranous structures called replication organelles (ROs). Mechanistic studies of RO formation have been difficult because perturbations affecting viral replication have an impact on viral protein amounts, thus affecting RO biogenesis. Here, we present a detailed guide on how to use a replication-independent expression system, designated pIRO (plasmid-induced replication organelle formation), inducing bona fide flavivirus ROs in transfected cells. This will be useful for mechanistic studies of viral and cellular factors driving flavivirus RO biogenesis. For complete details on the use and execution of this protocol, please refer to Cerikan et al. (2020).

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:1

Enthalten in:

STAR protocols - 1(2020), 3 vom: 18. Dez., Seite 100173

Sprache:

Englisch

Beteiligte Personen:

Goellner, Sarah [VerfasserIn]
Cerikan, Berati [VerfasserIn]
Cortese, Mirko [VerfasserIn]
Neufeldt, Christopher J [VerfasserIn]
Haselmann, Uta [VerfasserIn]
Bartenschlager, Ralf [VerfasserIn]

Links:

Volltext

Themen:

Cell Biology
Journal Article
Microscopy
Molecular Biology
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 09.03.2022

Date Revised 09.03.2022

published: Electronic-eCollection

Citation Status MEDLINE

doi:

10.1016/j.xpro.2020.100173

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM319412032