Spinach and Chive for Kidney Tubule Engineering : the Limitations of Decellularized Plant Scaffolds and Vasculature

Tissue decellularization yields complex scaffolds with retained composition and structure, and plants offer an inexhaustible natural source of numerous shapes. Plant tissue could be a solution for regenerative organ replacement strategies and advanced in vitro modeling, as biofunctionalization of decellularized tissue allows adhesion of various kinds of human cells that can grow into functional tissue. Here, we investigated the potential of spinach leaf vasculature and chive stems for kidney tubule engineering to apply in tubular transport studies. We successfully decellularized both plant tissues and confirmed general scaffold suitability for topical recellularization with renal cells. However, due to anatomical restrictions, we believe that spinach and chive vasculature themselves cannot be recellularized by current methods. Moreover, gradual tissue disintegration and deficient diffusion capacity make decellularized plant scaffolds unsuitable for kidney tubule engineering, which relies on transepithelial solute exchange between two compartments. We conclude that plant-derived structures and biomaterials need to be carefully considered and possibly integrated with other tissue engineering technologies for enhanced capabilities.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:23

Enthalten in:

The AAPS journal - 23(2020), 1 vom: 28. Dez., Seite 11

Sprache:

Englisch

Beteiligte Personen:

Jansen, Katja [VerfasserIn]
Evangelopoulou, Marianna [VerfasserIn]
Pou Casellas, Carla [VerfasserIn]
Abrishamcar, Sarina [VerfasserIn]
Jansen, Jitske [VerfasserIn]
Vermonden, Tina [VerfasserIn]
Masereeuw, Rosalinde [VerfasserIn]

Links:

Volltext

Themen:

Biocompatible Materials
Decellularization
Journal Article
Plant scaffolds
Proximal tubule
Regenerative medicine
Research Support, Non-U.S. Gov't
Tissue engineering

Anmerkungen:

Date Completed 27.09.2021

Date Revised 27.09.2021

published: Electronic

Citation Status MEDLINE

doi:

10.1208/s12248-020-00550-0

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM319339378