Influence of fuel oil on Platymonas helgolandica : An acute toxicity evaluation to amino acids
Copyright © 2020 Elsevier Ltd. All rights reserved..
It is highly likely that the toxicity of water accommodated fractions (WAF) will influence marine microalgae, and consequently lead to potential risk for the marine ecological environment. However, it was often neglected whether WAF can influence the transformation of relative compounds in organisms. The metabolism of amino acids (AAs) can be used to track physiological changes in microalgae because amino acids are the basis of proteins and enzymes. In this study, using marine Chlorophyta Platymonas helgolandica as the test organism, the effects of different concentrations of WAF on AA compositions and stable carbon isotope ratios (δ13C) of individual AAs of Platymonas helgolandica were investigated. The results showed that the WAF of #180 fuel oil had an obvious suppressing effect on the growth and chlorophyll a content of microalgae. The growth inhibitory rate at 96 h was 80.66% at a WAF concentration of 0.50 mg L-1 compared with the control. Furthermore, seven among the 16 AAs, including alanine, cysteine, proline, aspartic acid, lysine, histidine and tyrosine, had relatively high abundance. Under the glycolysis pathway, the cysteine abundance was higher than control, meaning that the biosynthesized pathway of alanine through cysteine as a precursor could be damaged. Phosphoenolpyruvate (PEP) was an important synthesis precursor of alanine (leucine) and aromatic AA family (Phenylalanine and tyrosine), and played an important role in δ13CAAs fractionation under the WAF stress. Under the TCA pathway, to protect cell metabolism activities under WAF stress, the δ13C value of threonine and proline abundance in microalgae with the increase in WAF stress. Therefore, δ13CAAs fractionation can be used as a novel method for toxicity evaluation of WAF on future.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:271 |
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| Enthalten in: |
Environmental pollution (Barking, Essex : 1987) - 271(2021) vom: 15. Feb., Seite 116226 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Na [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 03.02.2021 Date Revised 03.02.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.envpol.2020.116226 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM319246795 |
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| 245 | 1 | 0 | |a Influence of fuel oil on Platymonas helgolandica |b An acute toxicity evaluation to amino acids |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier Ltd. All rights reserved. | ||
| 520 | |a It is highly likely that the toxicity of water accommodated fractions (WAF) will influence marine microalgae, and consequently lead to potential risk for the marine ecological environment. However, it was often neglected whether WAF can influence the transformation of relative compounds in organisms. The metabolism of amino acids (AAs) can be used to track physiological changes in microalgae because amino acids are the basis of proteins and enzymes. In this study, using marine Chlorophyta Platymonas helgolandica as the test organism, the effects of different concentrations of WAF on AA compositions and stable carbon isotope ratios (δ13C) of individual AAs of Platymonas helgolandica were investigated. The results showed that the WAF of #180 fuel oil had an obvious suppressing effect on the growth and chlorophyll a content of microalgae. The growth inhibitory rate at 96 h was 80.66% at a WAF concentration of 0.50 mg L-1 compared with the control. Furthermore, seven among the 16 AAs, including alanine, cysteine, proline, aspartic acid, lysine, histidine and tyrosine, had relatively high abundance. Under the glycolysis pathway, the cysteine abundance was higher than control, meaning that the biosynthesized pathway of alanine through cysteine as a precursor could be damaged. Phosphoenolpyruvate (PEP) was an important synthesis precursor of alanine (leucine) and aromatic AA family (Phenylalanine and tyrosine), and played an important role in δ13CAAs fractionation under the WAF stress. Under the TCA pathway, to protect cell metabolism activities under WAF stress, the δ13C value of threonine and proline abundance in microalgae with the increase in WAF stress. Therefore, δ13CAAs fractionation can be used as a novel method for toxicity evaluation of WAF on future | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Amino acids | |
| 650 | 4 | |a Compound specific stable carbon isotope | |
| 650 | 4 | |a Platymonas helgolandica | |
| 650 | 4 | |a Water accommodated fractions | |
| 650 | 7 | |a Amino Acids |2 NLM | |
| 650 | 7 | |a Fuel Oils |2 NLM | |
| 650 | 7 | |a Petroleum |2 NLM | |
| 650 | 7 | |a Water Pollutants, Chemical |2 NLM | |
| 650 | 7 | |a Chlorophyll A |2 NLM | |
| 650 | 7 | |a YF5Q9EJC8Y |2 NLM | |
| 700 | 1 | |a Liu, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Zhengyu |e verfasserin |4 aut | |
| 700 | 1 | |a Lou, Yadi |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yuxin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Xinda |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Guoguang |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:271 |g year:2021 |g day:15 |g month:02 |g pages:116226 |
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