Redox regulation of glutathione peroxidase by thioredoxin in longan fruit in relation to senescence and quality deterioration
Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved..
Thioredoxins (Trxs) are important redox regulators in organisms. However, their involvement in fruit senescence and quality deterioration remains unclear. In this study, one Trx (DlTrx1) and one NADPH-dependent Trx reductase (DlNRT1) cDNAs, were cloned from longan fruit. The DlTrx1 could be effectively reduced by the DlNTR1. Expression of DlTrx1 and DlNTR1 were up-regulated during fruit senescence and quality deterioration. We further identified 33 potential Trx target proteins in longan, including one glutathione peroxidase (DlGpx). DlTrx1 could physically interact with DlGpx. DlTrx1 in combination with DlNTR1 effectively activated DlGpx activity by regulating its redox state. Cys90 in DlGPx could form a disulfide bond with either Cys42 or Cys71, which were the sites of redox modulation. Furthermore, DlGpx exhibited a higher ratio of disulfide bonds to sulfhydryl groups in senescent or deteriorative fruit. We propose that Trx-mediated redox regulation of DlGpx is involved in senescence or quality deterioration of harvested longan fruit.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:345 |
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| Enthalten in: |
Food chemistry - 345(2021) vom: 30. Mai, Seite 128664 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wu, Fuwang [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 11.03.2021 Date Revised 11.03.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.foodchem.2020.128664 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM319057488 |
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| 520 | |a Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a Thioredoxins (Trxs) are important redox regulators in organisms. However, their involvement in fruit senescence and quality deterioration remains unclear. In this study, one Trx (DlTrx1) and one NADPH-dependent Trx reductase (DlNRT1) cDNAs, were cloned from longan fruit. The DlTrx1 could be effectively reduced by the DlNTR1. Expression of DlTrx1 and DlNTR1 were up-regulated during fruit senescence and quality deterioration. We further identified 33 potential Trx target proteins in longan, including one glutathione peroxidase (DlGpx). DlTrx1 could physically interact with DlGpx. DlTrx1 in combination with DlNTR1 effectively activated DlGpx activity by regulating its redox state. Cys90 in DlGPx could form a disulfide bond with either Cys42 or Cys71, which were the sites of redox modulation. Furthermore, DlGpx exhibited a higher ratio of disulfide bonds to sulfhydryl groups in senescent or deteriorative fruit. We propose that Trx-mediated redox regulation of DlGpx is involved in senescence or quality deterioration of harvested longan fruit | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Aril breakdown | |
| 650 | 4 | |a Glutathione peroxidase | |
| 650 | 4 | |a Quality deterioration | |
| 650 | 4 | |a Reactive oxygen species | |
| 650 | 4 | |a Redox regulation | |
| 650 | 4 | |a Thioredoxin | |
| 650 | 7 | |a Thioredoxins |2 NLM | |
| 650 | 7 | |a 52500-60-4 |2 NLM | |
| 650 | 7 | |a Glutathione Peroxidase |2 NLM | |
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| 650 | 7 | |a Glutathione |2 NLM | |
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| 700 | 1 | |a Jiang, Guoxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Huiling |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Lu |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Hanzhi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Dandan |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Yueming |e verfasserin |4 aut | |
| 700 | 1 | |a Duan, Xuewu |e verfasserin |4 aut | |
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