In Vivo Functional Analysis of Nonconserved Human lncRNAs Using a Humanized Mouse Model
LncRNAs (long noncoding RNAs) are transcripts that are at least 200 nucleotides long and lack any predicted coding potential. Whereas significant progress has been made in deciphering the function of mouse lncRNAs, critical gaps remain in understanding how human lncRNAs exercise their function in a physiological context. As most human lncRNAs are currently considered nonconserved and often do not have homologs in mouse, the technical bottleneck is the lack of a suitable model to study the physiological function. Chimeric mice with repopulated human hepatocytes have emerged as promising tools to study human-specific, liver enriched lncRNAs. Among all liver-specific humanized mouse models, TK-NOG is relatively easy to prepare and holds a higher repopulation rate for a prolonged period of time. In this chapter, we will illustrate how to establish humanized TK-NOG mice for in vivo analysis of human lncRNAs in detail.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2254 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2254(2021) vom: 16., Seite 339-347 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ma, Yonghe [VerfasserIn] |
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| Links: |
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| Themen: |
Humanized mice |
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| Anmerkungen: |
Date Completed 29.03.2021 Date Revised 14.10.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-1158-6_21 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM318911787 |
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| 245 | 1 | 0 | |a In Vivo Functional Analysis of Nonconserved Human lncRNAs Using a Humanized Mouse Model |
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| 500 | |a Date Revised 14.10.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a LncRNAs (long noncoding RNAs) are transcripts that are at least 200 nucleotides long and lack any predicted coding potential. Whereas significant progress has been made in deciphering the function of mouse lncRNAs, critical gaps remain in understanding how human lncRNAs exercise their function in a physiological context. As most human lncRNAs are currently considered nonconserved and often do not have homologs in mouse, the technical bottleneck is the lack of a suitable model to study the physiological function. Chimeric mice with repopulated human hepatocytes have emerged as promising tools to study human-specific, liver enriched lncRNAs. Among all liver-specific humanized mouse models, TK-NOG is relatively easy to prepare and holds a higher repopulation rate for a prolonged period of time. In this chapter, we will illustrate how to establish humanized TK-NOG mice for in vivo analysis of human lncRNAs in detail | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Intramural | |
| 650 | 4 | |a Humanized mice | |
| 650 | 4 | |a Liver | |
| 650 | 4 | |a Nonconserved | |
| 650 | 4 | |a Repopulation | |
| 650 | 4 | |a TK-NOG | |
| 650 | 4 | |a lncRNA | |
| 650 | 7 | |a RNA, Long Noncoding |2 NLM | |
| 700 | 1 | |a Jiang, Cheng-Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Haiming |e verfasserin |4 aut | |
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