Comparative Study of Senescent Th Biomarkers in Healthy Donors and Early Arthritis Patients. Analysis of VPAC Receptors and Their Influence

Pro-inflammatory CD4+CD28- T cells are characteristic of immunosenescence, but also of several autoimmune/inflammatory diseases. Vasoactive intestinal peptide (VIP) acts as an anti-inflammatory and immunomodulatory mediator on these cells. Our objective was to study the mutual influence between senescent Th cells and VIP axis in early arthritis (EA), comparing with non-EA donors. We characterized the correlation between senescent Th cells and clinic parameters of EA as well as the behavior of senescent Th biomarkers by real-time PCR. Clinical data were systematically recorded at baseline and after 6 months of follow-up. The number of CD4+CD28- T cells measured by sorting is higher in patients who initially meet ACR classification criteria for rheumatoid arthritis (RA) compared to those who were classified as undifferentiated arthritis (UA). A slight positive correlation between EA CD4+CD28- T cells and CRP or ESR and a negative correlation with bone mineral density were found. Th senescent biomarkers in EA CD4+CD28- T cells were similar to donors, however some of them increased after 6 months of follow-up. VPAC receptors were analyzed by real-time PCR and immunofluorescence, and CD4+CD28- T cells showed higher expression of VPAC2 and lower of VPAC1, VPAC2 showing a significant increased expression in EA cells. Sorted CD4+CD28- T cells were in vitro expanded in presence of VIP, wherein VIP increased senescent biomarker CD27, while it diminished CD57 or NKG2 senescent biomarkers. Our study demonstrates for the first time the existence of a link between senescent Th cells and the VIP axis.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:9

Enthalten in:

Cells - 9(2020), 12 vom: 04. Dez.

Sprache:

Englisch

Beteiligte Personen:

Villanueva-Romero, Raúl [VerfasserIn]
Lamana, Amalia [VerfasserIn]
Flores-Santamaría, Marissa [VerfasserIn]
Carrión, Mar [VerfasserIn]
Pérez-García, Selene [VerfasserIn]
Triguero-Martínez, Ana [VerfasserIn]
Tomero, Eva [VerfasserIn]
Criado, Gabriel [VerfasserIn]
Pablos, José L [VerfasserIn]
González-Álvaro, Isidoro [VerfasserIn]
Martínez, Carmen [VerfasserIn]
Juarranz, Yasmina [VerfasserIn]
Gomariz, Rosa P [VerfasserIn]
Gutiérrez-Cañas, Irene [VerfasserIn]

Links:

Volltext

Themen:

37221-79-7
Biomarkers
CD28 Antigens
CD4+CD28− T cells
CD57 Antigens
Comparative Study
Early arthritis
Journal Article
Research Support, Non-U.S. Gov't
Rheumatoid arthritis
Senescent Th cells
VIP
VPAC receptors
Vasoactive Intestinal Peptide

Anmerkungen:

Date Completed 09.07.2021

Date Revised 09.07.2021

published: Electronic

Citation Status MEDLINE

doi:

10.3390/cells9122592

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM318571455