Effects of Inonotus obliquus Polysaccharides on Proliferation, Invasion, Migration, and Apoptosis of Osteosarcoma Cells

Copyright © 2020 Baohui Su et al..

OBJECTIVES: To observe the effect of Inonotus obliquus polysaccharide (IOP) on the proliferation, invasion, migration, and apoptosis of osteosarcoma cells and to elucidate its underlying molecular mechanism.

METHODS: IOP was extracted from Inonotus obliquus, human osteosarcoma MG-63 cells and U2OS cells were cultured in vitro, and the effects of IOP on the proliferation, migration, invasion, and apoptosis of MG-63 cells and U2OS cells were determined by CCK-8 assays, cell scratch assays, transwell assays, and flow cytometry, respectively. Western blot was used to detect the expression of related proteins in the Akt/mTOR and NF-κB signaling pathways.

RESULTS: Compared with the control group, MG-63 cells and U2OS cells treated with IOP of 80 μg/ml, 160 μg/ml, and 320 μ g/ml in the experimental group had significantly lower proliferation activity, decreased migration and invasion ability, and increased apoptosis rate (P < 0.05). Furthermore, IOP could significantly inhibit the activation of the Akt/mTOR and NF-κB signaling pathway (P < 0.05).

CONCLUSION: IOP can regulate the proliferation, migration, invasion, and apoptosis of osteosarcoma cells by inhibiting the activation of the Akt/mTOR signaling pathway. It has antitumor activity on osteosarcoma and has the potential of clinical application in osteosarcoma treatment.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:2020

Enthalten in:

Analytical cellular pathology (Amsterdam) - 2020(2020) vom: 26., Seite 4282036

Sprache:

Englisch

Beteiligte Personen:

Su, Baohui [VerfasserIn]
Yan, Xuezhi [VerfasserIn]
Li, Yuezhong [VerfasserIn]
Zhang, Junshan [VerfasserIn]
Xia, Xiaoyan [VerfasserIn]

Links:

Volltext

Themen:

EC 2.7.11.1
Journal Article
NF-kappa B
Polysaccharides
Proto-Oncogene Proteins c-akt
TOR Serine-Threonine Kinases

Anmerkungen:

Date Completed 05.08.2021

Date Revised 18.04.2022

published: Electronic-eCollection

Citation Status MEDLINE

doi:

10.1155/2020/4282036

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM318485125