MicroRNA-23 regulates the growth, metastasis and chemosensitivity of human gastric cancer cells by targeting MAP3K9

PURPOSE: The efficient therapeutic targets which could be utilized for gastric cancer are limited. In this context, this study was undertaken to evaluate possible anticancer activity of miR-23 against gastric cancer.

METHODS: The normal GES-1 and human AGS, SNU-1 and SNU-5 gastric cancer cells were used in this study. qRT-PCR was used for the determination of miR-23 expression. MTT assay was used for cell viability and acridine orange (AO)/ethidium bromide (EB) assay was used for detection of apoptosis. The experiments were performed in triplicate.

RESULTS: The microRNA (miR)-23 was downregulated in gastric cancer cells and showed inhibitory effect on cell growth which was manifested as decline in cell survival. Additionally, the chemosensitivity of gastric cancer cells to cisplatin was enhanced with miR-23 overexpression. Furthermore, miR-23 also inhibited the migration and invasion of cancer cells. MAP3K9 was shown to be the target gene of miR-23 and silencing of MAP3K9 was seen to mimic the growth inhibitory effect of miR-23. Overexpression of MAP3K9 reversed the growth inhibition in miR-23 mimics transfected gastric cancer cells.

CONCLUSION: miR-23 exerts growth inhibitory effect against gastric cancer cells and negatively regulates the cell migration and invasion along with enhancement of chemosensitivity of cancer cells.

Errataetall:

RetractionIn: J BUON. 2021 Jul-Aug;26(4):1695. - PMID 34565051

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:25

Enthalten in:

Journal of B.U.ON. : official journal of the Balkan Union of Oncology - 25(2020), 5 vom: 01. Sept., Seite 2315-2321

Sprache:

Englisch

Beteiligte Personen:

Li, Deping [VerfasserIn]
Wang, Juan [VerfasserIn]
Hui, Yuanjian [VerfasserIn]
Yang, Yan [VerfasserIn]
Peng, Xingchun [VerfasserIn]
Li, Shengbao [VerfasserIn]
Sun, Chuantao [VerfasserIn]

Themen:

EC 2.7.11.25
Journal Article
MAP Kinase Kinase Kinases
MAP3K9 protein, human
MIRN23a microRNA, human
MicroRNAs
Retracted Publication

Anmerkungen:

Date Completed 20.08.2021

Date Revised 26.09.2021

published: Print

RetractionIn: J BUON. 2021 Jul-Aug;26(4):1695. - PMID 34565051

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM318438399