CaSBP11 Participates in the Defense Response of Pepper to Phytophthora capsici through Regulating the Expression of Defense-Related Genes

Squamosa promoter binding protein (SBP)-box genes are plant-specific transcription factors involved in plant growth and development, morphogenesis and biotic and abiotic stress responses. However, these genes have been understudied in pepper, especially with respect to defense responses to Phytophthora capsici infection. CaSBP11 is a SBP-box family gene in pepper that was identified in our previous research. Silencing CaSBP11 enhanced the defense response of pepper plants to Phytophthora capsici. Without treatment, the expression of defense-related genes (CaBPR1, CaPO1, CaSAR8.2 and CaDEF1) increased in CaSBP11-silenced plants. However, the expression levels of these genes were inhibited under transient CaSBP11 expression. CaSBP11 overexpression in transgenic Nicotiana benthamiana decreased defense responses, while in Arabidopsis, it induced or inhibited the expression of genes in the salicylic acid and jasmonic acid signaling pathways. CaSBP11 overexpression in sid2-2 mutants induced AtNPR1, AtNPR3, AtNPR4, AtPAD4, AtEDS1, AtEDS5, AtMPK4 and AtNDR1 expression, while AtSARD1 and AtTGA6 expression was inhibited. CaSBP11 overexpression in coi1-21 and coi1-22 mutants, respectively, inhibited AtPDF1.2 expression and induced AtPR1 expression. These results indicate CaSBP11 has a negative regulatory effect on defense responses to Phytophthora capsici. Moreover, it may participate in the defense response of pepper to Phytophthora capsici by regulating defense-related genes and the salicylic and jasmonic acid-mediated disease resistance signaling pathways.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:21

Enthalten in:

International journal of molecular sciences - 21(2020), 23 vom: 28. Nov.

Sprache:

Englisch

Beteiligte Personen:

Zhang, Huai-Xia [VerfasserIn]
Feng, Xiao-Hui [VerfasserIn]
Jin, Jing-Hao [VerfasserIn]
Khan, Abid [VerfasserIn]
Guo, Wei-Li [VerfasserIn]
Du, Xiao-Hua [VerfasserIn]
Gong, Zhen-Hui [VerfasserIn]

Links:

Volltext

Themen:

6RI5N05OWW
CaSBP11
Cyclopentanes
Defense-related genes
Jasmonic acid
Journal Article
Nicotiana benthamiana
Oxylipins
Pepper
Phytophthora capsici
Plant Proteins

Anmerkungen:

Date Completed 03.03.2021

Date Revised 13.12.2023

published: Electronic

Citation Status MEDLINE

doi:

10.3390/ijms21239065

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM318269325