B Cell Epitopes of Four Fimbriae Antigens of Klebsiella pneumoniae : A Comprehensive In Silico Study for Vaccine Development

© Springer Nature B.V. 2020..

Klebsiella pneumoniae is one of the major causes of nosocomial infections worldwide which can cause several diseases in children and adults. The globally dissemination of hyper-virulent strains of K. pneumoniae and the emergence of antibiotics-resistant isolates of this pathogen narrows down the treatment options and has renewed interest in its vaccines. Vaccine candidates of Klebsiella pneumoniae have not been adequately protective, safe and globally available yet. In K. pneumoniae infection, it is well known that B cells that induce robust humoral immunity are necessary for the host complete protection. Identifying the B cell epitopes of antigens is valuable to design novel vaccine candidates. In the present study using immunoinformatics approaches we found B cell epitopes of four K. pneumoniae type 1 fimbriae antigens namely FimA, FimF, FimG, and FimH. Linear and conformational B cell epitopes of each antigen were predicted using different programs. Subsequently, many bioinformatics assays were applied to choose the best epitopes including prediction antigenicity, toxicity, human similarity and investigation on experimental records. These assays resulted in final four epitopes (each for one Fim protein). These final epitopes were modeled and their physiochemical properties were estimated to be used as potential vaccine candidates. Altogether, we found four B cell epitopes of K. pneumoniae Fim antigens that are immunogen, antigenic, not similar to human peptides, not allergen and not toxic. Also, they have suitable physiochemical properties to administrate as vaccine, although their complete efficacy should be also shown in vitro and in vivo.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:27

Enthalten in:

International journal of peptide research and therapeutics - 27(2021), 2 vom: 10., Seite 875-886

Sprache:

Englisch

Beteiligte Personen:

Zargaran, Fatemeh Nemati [VerfasserIn]
Akya, Alisha [VerfasserIn]
Rezaeian, Shahab [VerfasserIn]
Ghadiri, Keyghobad [VerfasserIn]
Lorestani, Roya Chegene [VerfasserIn]
Madanchi, Hamid [VerfasserIn]
Safaei, Sadegh [VerfasserIn]
Rostamian, Mosayeb [VerfasserIn]

Links:

Volltext

Themen:

B cell epitopes
Immunoinformatics
Journal Article
Klebsiella pneumoniae
Vaccine

Anmerkungen:

Date Revised 18.02.2022

published: Print-Electronic

Citation Status PubMed-not-MEDLINE

doi:

10.1007/s10989-020-10134-3

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM318170701