The role of ATF6 in Cr(VI)-induced apoptosis in DF-1 cells
Copyright © 2020 Elsevier B.V. All rights reserved..
Hexavalent chromium (Cr(VI)) is a common heavy metal pollutant in environment and has been proved possessing the cytotoxicity. In this study, we aimed to investigate the role of activating transcription factor 6 (ATF-6) in apoptosis of chicken embryo fibroblasts cell line (DF-1) induced by Cr(VI). Firstly, DF-1 cells were exposed to Cr(VI) to establish the cytotoxicity model, then the cell apoptosis and ATF-6 protein level were analyzed. By silencing ATF-6 gene, changes of the apoptosis rate and apoptotic proteins were examined. To further explore the regulatory mechanism of ATF-6, endoplasmic reticulum (ER) stress, mitochondrial function, reactive oxygen species (ROS) level, as well as the related pathway were evaluated. Results showed that Cr(VI) can result in DF-1 cell apoptosis, along with mitochondrial membrane potential (MMP) reducing and ER stress. Meanwhile, ATF-6 silencing lowered the apoptosis rate and ER stress level, showing with the decrease of XBP-1, PERK, GRP78, Caspase-12, Cleaved Caspase-3 and the increase of Bcl-2. Further analysis found that ATF-6 silencing down-regulated ROS and caused MMP loss, suggesting that ATF-6 silencing inhibited Cr(VI)-induced mitochondrial damage. In conclusion, this study indicate that ATF-6 plays an important regulatory role in Cr(VI)-induced DF-1 cell apoptosis through the ER stress and mitochondrial pathway.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:410 |
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| Enthalten in: |
Journal of hazardous materials - 410(2021) vom: 15. Mai, Seite 124607 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Shuo [VerfasserIn] |
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| Links: |
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| Themen: |
0R0008Q3JB |
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| Anmerkungen: |
Date Completed 31.05.2021 Date Revised 31.05.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jhazmat.2020.124607 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM318101459 |
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| 100 | 1 | |a Zhang, Shuo |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The role of ATF6 in Cr(VI)-induced apoptosis in DF-1 cells |
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| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a Hexavalent chromium (Cr(VI)) is a common heavy metal pollutant in environment and has been proved possessing the cytotoxicity. In this study, we aimed to investigate the role of activating transcription factor 6 (ATF-6) in apoptosis of chicken embryo fibroblasts cell line (DF-1) induced by Cr(VI). Firstly, DF-1 cells were exposed to Cr(VI) to establish the cytotoxicity model, then the cell apoptosis and ATF-6 protein level were analyzed. By silencing ATF-6 gene, changes of the apoptosis rate and apoptotic proteins were examined. To further explore the regulatory mechanism of ATF-6, endoplasmic reticulum (ER) stress, mitochondrial function, reactive oxygen species (ROS) level, as well as the related pathway were evaluated. Results showed that Cr(VI) can result in DF-1 cell apoptosis, along with mitochondrial membrane potential (MMP) reducing and ER stress. Meanwhile, ATF-6 silencing lowered the apoptosis rate and ER stress level, showing with the decrease of XBP-1, PERK, GRP78, Caspase-12, Cleaved Caspase-3 and the increase of Bcl-2. Further analysis found that ATF-6 silencing down-regulated ROS and caused MMP loss, suggesting that ATF-6 silencing inhibited Cr(VI)-induced mitochondrial damage. In conclusion, this study indicate that ATF-6 plays an important regulatory role in Cr(VI)-induced DF-1 cell apoptosis through the ER stress and mitochondrial pathway | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a ATF-6 | |
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a ER stress | |
| 650 | 4 | |a Hexavalent chromium | |
| 650 | 4 | |a Mitochondria | |
| 650 | 7 | |a Reactive Oxygen Species |2 NLM | |
| 650 | 7 | |a Chromium |2 NLM | |
| 650 | 7 | |a 0R0008Q3JB |2 NLM | |
| 650 | 7 | |a chromium hexavalent ion |2 NLM | |
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| 700 | 1 | |a Zhao, Xiaona |e verfasserin |4 aut | |
| 700 | 1 | |a Hao, Jiajia |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Yiran |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yue |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Lumei |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Shuhua |e verfasserin |4 aut | |
| 700 | 1 | |a Yi, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yongxia |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jianzhu |e verfasserin |4 aut | |
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