Cellular and Biochemical Analysis of Bronchoalveolar Lavage Fluid from Murine Lungs

Bronchoalveolar lavage (BAL) is a technique used to collect the contents of the airways. The fluid recovered, called BAL fluid (BALF), serves as a dynamic tool to identify various disease pathologies ranging from asthma to infectious diseases to cancer in the lungs. A wide array of tests can be performed with BALF, including total and differential leukocyte counts (DLC), enzyme-linked immunosorbent assays (ELISA) or flow-cytometric quantitation of inflammatory mediators, such as cytokines, chemokines and adhesion molecules, and assessment of nitrate and nitrite content for estimation of nitric oxide synthase (NOS) activity. Here, we describe a detailed procedure for the collection of BALF for a variety of downstream usages, including DLC by cytological and flow-cytometry-based methods, multiplex cytokine analysis by flow cytometry, and NOS activity analysis by determining nitrate and nitrite levels.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:2223

Enthalten in:

Methods in molecular biology (Clifton, N.J.) - 2223(2021) vom: 23., Seite 201-215

Sprache:

Englisch

Beteiligte Personen:

Kalidhindi, Rama Satyanarayana Raju [VerfasserIn]
Ambhore, Nilesh Sudhakar [VerfasserIn]
Sathish, Venkatachalem [VerfasserIn]

Links:

Volltext

Themen:

Cytokines
Differential leukocyte count
EC 1.14.13.39
Flow cytometry
Journal Article
Nitric Oxide Synthase
Nitric oxide synthase
Research Support, N.I.H., Extramural
Tracheostomy

Anmerkungen:

Date Completed 26.03.2021

Date Revised 02.01.2022

published: Print

Citation Status MEDLINE

doi:

10.1007/978-1-0716-1001-5_15

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM317935666