Cellular and Biochemical Analysis of Bronchoalveolar Lavage Fluid from Murine Lungs
Bronchoalveolar lavage (BAL) is a technique used to collect the contents of the airways. The fluid recovered, called BAL fluid (BALF), serves as a dynamic tool to identify various disease pathologies ranging from asthma to infectious diseases to cancer in the lungs. A wide array of tests can be performed with BALF, including total and differential leukocyte counts (DLC), enzyme-linked immunosorbent assays (ELISA) or flow-cytometric quantitation of inflammatory mediators, such as cytokines, chemokines and adhesion molecules, and assessment of nitrate and nitrite content for estimation of nitric oxide synthase (NOS) activity. Here, we describe a detailed procedure for the collection of BALF for a variety of downstream usages, including DLC by cytological and flow-cytometry-based methods, multiplex cytokine analysis by flow cytometry, and NOS activity analysis by determining nitrate and nitrite levels.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2021 |
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Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:2223 |
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Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2223(2021) vom: 23., Seite 201-215 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Kalidhindi, Rama Satyanarayana Raju [VerfasserIn] |
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Links: |
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Themen: |
Cytokines |
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Anmerkungen: |
Date Completed 26.03.2021 Date Revised 02.01.2022 published: Print Citation Status MEDLINE |
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doi: |
10.1007/978-1-0716-1001-5_15 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM317935666 |
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