The role of combined BRAFV600E gene detection in the diagnosis of thyroid nodule determined as Bethesda Ⅲ by fine-needle aspiration
Objective: To analyze the malignant probability of thyroid nodules with the diagnosis of atypia of undetermined significance or follicular lesion of undetermined significance (AUS/FLUS) determined by fine-needle aspiration (FNA) and to explore the value of the combined application of BRAFV600E gene detection for the diagnosis of benign and malignant thyroid nodules. Methods: A total of 114 patients including 20 males and 94 females, aged 16-76 years old with thyroid nodules underwent FNA examination and surgical treatment in the Affiliated Cancer Hospital of Zhengzhou University from October 2018 to November 2019 were retrospectively analyzed. Postoperative histopathological results were used as the gold standard for the diagnosis of malignant thyroid nodules. The malignant rate of thyroid nodules with the diagnosis of AUS/FLUS was evaluated. Differential diagnostic efficacy of preoperative FNA combined with BRAFV600E gene detection for papillary thyroid carcinoma (PTC) was analyzed by McNemer test and diagnostic test evaluation method. Results: The mutation rate of BRAFV600E gene was 84.76% (89/105) in PTC. PTC accounted for 57.14% (12/21) of the patients with the diagnoses of AUS/FLUS determined by FNA. The specificity, sensitivity, positive predictive value and negative predictive value of BRAFV600E mutation examination for the diagnosis of malignant thyroid nodules determined preoperatively as AUS/FLUS were 9/9, 5/12, 5/5 and 9/16, respectively. BRAFV600E mutation examination could improve the detection rate of PTC in patients with AUS/FLUS (OR=0.438, 95%CI=0.251-0.763, P=0.016). Conclusion: FNA combined with BRAFV600E mutation examination can significantly improve the detection rate of malignant thyroid nodules diagnosed preoperatively as AUS/FLUS.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2020 |
---|---|
Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:55 |
---|---|
Enthalten in: |
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery - 55(2020), 11 vom: 07. Nov., Seite 1057-1062 |
Sprache: |
Chinesisch |
---|
Beteiligte Personen: |
Zhou, B [VerfasserIn] |
---|
Links: |
---|
Themen: |
BRAF gene |
---|
Anmerkungen: |
Date Completed 21.12.2020 Date Revised 21.12.2020 published: Print Citation Status MEDLINE |
---|
doi: |
10.3760/cma.j.cn115330-20200509-00390 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM317780352 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM317780352 | ||
003 | DE-627 | ||
005 | 20231225164001.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2020 xx |||||o 00| ||chi c | ||
024 | 7 | |a 10.3760/cma.j.cn115330-20200509-00390 |2 doi | |
028 | 5 | 2 | |a pubmed24n1059.xml |
035 | |a (DE-627)NLM317780352 | ||
035 | |a (NLM)33210886 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a chi | ||
100 | 1 | |a Zhou, B |e verfasserin |4 aut | |
245 | 1 | 4 | |a The role of combined BRAFV600E gene detection in the diagnosis of thyroid nodule determined as Bethesda Ⅲ by fine-needle aspiration |
264 | 1 | |c 2020 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 21.12.2020 | ||
500 | |a Date Revised 21.12.2020 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Objective: To analyze the malignant probability of thyroid nodules with the diagnosis of atypia of undetermined significance or follicular lesion of undetermined significance (AUS/FLUS) determined by fine-needle aspiration (FNA) and to explore the value of the combined application of BRAFV600E gene detection for the diagnosis of benign and malignant thyroid nodules. Methods: A total of 114 patients including 20 males and 94 females, aged 16-76 years old with thyroid nodules underwent FNA examination and surgical treatment in the Affiliated Cancer Hospital of Zhengzhou University from October 2018 to November 2019 were retrospectively analyzed. Postoperative histopathological results were used as the gold standard for the diagnosis of malignant thyroid nodules. The malignant rate of thyroid nodules with the diagnosis of AUS/FLUS was evaluated. Differential diagnostic efficacy of preoperative FNA combined with BRAFV600E gene detection for papillary thyroid carcinoma (PTC) was analyzed by McNemer test and diagnostic test evaluation method. Results: The mutation rate of BRAFV600E gene was 84.76% (89/105) in PTC. PTC accounted for 57.14% (12/21) of the patients with the diagnoses of AUS/FLUS determined by FNA. The specificity, sensitivity, positive predictive value and negative predictive value of BRAFV600E mutation examination for the diagnosis of malignant thyroid nodules determined preoperatively as AUS/FLUS were 9/9, 5/12, 5/5 and 9/16, respectively. BRAFV600E mutation examination could improve the detection rate of PTC in patients with AUS/FLUS (OR=0.438, 95%CI=0.251-0.763, P=0.016). Conclusion: FNA combined with BRAFV600E mutation examination can significantly improve the detection rate of malignant thyroid nodules diagnosed preoperatively as AUS/FLUS | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a BRAF gene | |
650 | 4 | |a Biopsy, fine-needle | |
650 | 4 | |a Diagnosis | |
650 | 4 | |a Thyroid neoplasms | |
650 | 4 | |a Thyroid nodule | |
700 | 1 | |a Zhai, Y F |e verfasserin |4 aut | |
700 | 1 | |a Zhang, D Y |e verfasserin |4 aut | |
700 | 1 | |a Wang, D Q |e verfasserin |4 aut | |
700 | 1 | |a Wei, L |e verfasserin |4 aut | |
700 | 1 | |a Qin, J W |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery |d 2005 |g 55(2020), 11 vom: 07. Nov., Seite 1057-1062 |w (DE-627)NLM155497294 |x 1673-0860 |7 nnns |
773 | 1 | 8 | |g volume:55 |g year:2020 |g number:11 |g day:07 |g month:11 |g pages:1057-1062 |
856 | 4 | 0 | |u http://dx.doi.org/10.3760/cma.j.cn115330-20200509-00390 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 55 |j 2020 |e 11 |b 07 |c 11 |h 1057-1062 |