High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures
© 2020, Minn et al..
During mammalian gastrulation, germ layers arise and are shaped into the body plan while extraembryonic layers sustain the embryo. Human embryonic stem cells, cultured with BMP4 on extracellular matrix micro-discs, reproducibly differentiate into gastruloids, expressing markers of germ layers and extraembryonic cells in radial arrangement. Using single-cell RNA sequencing and cross-species comparisons with mouse, cynomolgus monkey gastrulae, and post-implantation human embryos, we reveal that gastruloids contain cells transcriptionally similar to epiblast, ectoderm, mesoderm, endoderm, primordial germ cells, trophectoderm, and amnion. Upon gastruloid dissociation, single cells reseeded onto micro-discs were motile and aggregated with the same but segregated from distinct cell types. Ectodermal cells segregated from endodermal and extraembryonic but mixed with mesodermal cells. Our work demonstrates that the gastruloid system models primate-specific features of embryogenesis, and that gastruloid cells exhibit evolutionarily conserved sorting behaviors. This work generates a resource for transcriptomes of human extraembryonic and embryonic germ layers differentiated in a stereotyped arrangement.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
eLife - 9(2020) vom: 18. Nov. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Minn, Kyaw Thu [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 16.03.2021 Date Revised 30.03.2024 published: Electronic GEO: GSE144897, GSE130114, GSE74767, GSE125616, GSE136447, GSE75748, GSE85066, GSE109979, GSE140021, GSE134571 Citation Status MEDLINE |
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| doi: |
10.7554/eLife.59445 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM31773265X |
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| 100 | 1 | |a Minn, Kyaw Thu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures |
| 264 | 1 | |c 2020 | |
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| 500 | |a Date Revised 30.03.2024 | ||
| 500 | |a published: Electronic | ||
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020, Minn et al. | ||
| 520 | |a During mammalian gastrulation, germ layers arise and are shaped into the body plan while extraembryonic layers sustain the embryo. Human embryonic stem cells, cultured with BMP4 on extracellular matrix micro-discs, reproducibly differentiate into gastruloids, expressing markers of germ layers and extraembryonic cells in radial arrangement. Using single-cell RNA sequencing and cross-species comparisons with mouse, cynomolgus monkey gastrulae, and post-implantation human embryos, we reveal that gastruloids contain cells transcriptionally similar to epiblast, ectoderm, mesoderm, endoderm, primordial germ cells, trophectoderm, and amnion. Upon gastruloid dissociation, single cells reseeded onto micro-discs were motile and aggregated with the same but segregated from distinct cell types. Ectodermal cells segregated from endodermal and extraembryonic but mixed with mesodermal cells. Our work demonstrates that the gastruloid system models primate-specific features of embryogenesis, and that gastruloid cells exhibit evolutionarily conserved sorting behaviors. This work generates a resource for transcriptomes of human extraembryonic and embryonic germ layers differentiated in a stereotyped arrangement | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a amnion | |
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| 700 | 1 | |a He, Shenghua |e verfasserin |4 aut | |
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| 700 | 1 | |a George, Steven C |e verfasserin |4 aut | |
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| 700 | 1 | |a Morris, Samantha A |e verfasserin |4 aut | |
| 700 | 1 | |a Solnica-Krezel, Lilianna |e verfasserin |4 aut | |
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