Arecoline enhances miR-21 to promote buccal mucosal fibroblasts activation
Copyright © 2020. Published by Elsevier B.V..
BACKGROUND/PURPOSE: Oral submucous fibrosis (OSF) is an irreversible fibrosis disease and a potentially malignant disorder in the oral cavity. Various studies have shown that miR-21 was implicated in the fibrogenesis and carcinogenesis, but its functional role in the development of OSF has not been investigated.
METHODS: The expression levels of miR-21 in arecoline-stimulated normal buccal mucosal fibroblasts (BMFs) and OSF specimens were determined by qRT-PCR. Exogenous administration of TGF-β and its inhibitor (SB431542) were utilized to examine the involvement of TGF-β signaling in miR-21 alteration. Collagen gel contraction, transwell migration, and invasion assays were used to assess the myofibroblast activities. The relationship between α-SMA and miR-21 was calculated using the Pearson correlation coefficient.
RESULTS: MiR-21 expression was induced in BMFs by arecoline treatment in a dose-dependent manner. Our results showed that this upregulation was mediated by TGF-β signaling. Subsequently, we demonstrated that the administration of the miR-21 inhibitor suppressed the arecoline-induced myofibroblast characteristics, including a higher collagen gel contractility and cell motility, in normal BMFs. Furthermore, inhibition of miR-21 was sufficient to attenuate the myofibroblast features in fibrotic BMFs. Besides, we showed that the expression of miR-21 was aberrantly upregulated in the OSF tissues and there was a positive correlation between miR-21 and myofibroblast marker, α-SMA.
CONCLUSION: MiR-21 overexpression in OSF may be due to the stimulation of areca nut, which was mediated by the TGF-β pathway. Our data suggested that the repression of miR-21 was a promising direction to palliate the development and progression of OSF.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:120 |
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| Enthalten in: |
Journal of the Formosan Medical Association = Taiwan yi zhi - 120(2021), 4 vom: 01. Apr., Seite 1108-1113 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yang, Hui-Wen [VerfasserIn] |
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| Links: |
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| Themen: |
4ALN5933BH |
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| Anmerkungen: |
Date Completed 18.03.2021 Date Revised 18.03.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jfma.2020.10.019 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM317584960 |
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| 245 | 1 | 0 | |a Arecoline enhances miR-21 to promote buccal mucosal fibroblasts activation |
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| 500 | |a Date Revised 18.03.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020. Published by Elsevier B.V. | ||
| 520 | |a BACKGROUND/PURPOSE: Oral submucous fibrosis (OSF) is an irreversible fibrosis disease and a potentially malignant disorder in the oral cavity. Various studies have shown that miR-21 was implicated in the fibrogenesis and carcinogenesis, but its functional role in the development of OSF has not been investigated | ||
| 520 | |a METHODS: The expression levels of miR-21 in arecoline-stimulated normal buccal mucosal fibroblasts (BMFs) and OSF specimens were determined by qRT-PCR. Exogenous administration of TGF-β and its inhibitor (SB431542) were utilized to examine the involvement of TGF-β signaling in miR-21 alteration. Collagen gel contraction, transwell migration, and invasion assays were used to assess the myofibroblast activities. The relationship between α-SMA and miR-21 was calculated using the Pearson correlation coefficient | ||
| 520 | |a RESULTS: MiR-21 expression was induced in BMFs by arecoline treatment in a dose-dependent manner. Our results showed that this upregulation was mediated by TGF-β signaling. Subsequently, we demonstrated that the administration of the miR-21 inhibitor suppressed the arecoline-induced myofibroblast characteristics, including a higher collagen gel contractility and cell motility, in normal BMFs. Furthermore, inhibition of miR-21 was sufficient to attenuate the myofibroblast features in fibrotic BMFs. Besides, we showed that the expression of miR-21 was aberrantly upregulated in the OSF tissues and there was a positive correlation between miR-21 and myofibroblast marker, α-SMA | ||
| 520 | |a CONCLUSION: MiR-21 overexpression in OSF may be due to the stimulation of areca nut, which was mediated by the TGF-β pathway. Our data suggested that the repression of miR-21 was a promising direction to palliate the development and progression of OSF | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a MicroRNA-21 | |
| 650 | 4 | |a Myofibroblast | |
| 650 | 4 | |a Oral submucous fibrosis | |
| 650 | 4 | |a TGF-β pathway | |
| 650 | 7 | |a MIRN21 microRNA, human |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a Arecoline |2 NLM | |
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| 700 | 1 | |a Yu, Cheng-Chia |e verfasserin |4 aut | |
| 700 | 1 | |a Hsieh, Pei-Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Liao, Yi-Wen |e verfasserin |4 aut | |
| 700 | 1 | |a Chu, Pei-Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Chuan-Hang |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Chih-Yuan |e verfasserin |4 aut | |
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