Micromonospora fluminis sp. nov., isolated from mountain river sediment
During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38T was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus Micromonospora by means of a polyphasic taxonomy study. Strain A38T was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC15:0, 10 methyl C17:0, anteiso-C17:0 and iso-C17:0. The predominant menaquinones were MK-10(H4) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to Micromonospora tulbaghiae DSM 45142T (99.5 %), Micromonospora citrea DSM 43903T (99.4 %), Micromonospora marina DSM 45555T (99.4 %), Micromonospora maritima DSM 45782T (99.3 %), Micromonospora sediminicola DSM 45794T (99.3 %), Micromonospora aurantiaca DSM 43813T (99.2 %) and Micromonospora chaiyaphumensis DSM 45246T (99.2 %). The results of OrthoANIu analysis showed the highest similarity to Micromonospora chalcea DSM 43026T (96.4 %). However, the 16S rRNA and gyrB gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38T as a novel species. On the basis of the results presented here, we propose to classify strain A38T (=LMG 30467T=CECT 30034T) as the type strain of the novel species Micromonospora fluminis sp. nov.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:70 |
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| Enthalten in: |
International journal of systematic and evolutionary microbiology - 70(2020), 12 vom: 11. Dez., Seite 6428-6436 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Camacho Pozo, Miladis Isabel [VerfasserIn] |
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| Links: |
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| Themen: |
11032-49-8 |
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| Anmerkungen: |
Date Completed 11.01.2021 Date Revised 11.01.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.1099/ijsem.0.004554 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM317424599 |
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| 245 | 1 | 0 | |a Micromonospora fluminis sp. nov., isolated from mountain river sediment |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38T was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus Micromonospora by means of a polyphasic taxonomy study. Strain A38T was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC15:0, 10 methyl C17:0, anteiso-C17:0 and iso-C17:0. The predominant menaquinones were MK-10(H4) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to Micromonospora tulbaghiae DSM 45142T (99.5 %), Micromonospora citrea DSM 43903T (99.4 %), Micromonospora marina DSM 45555T (99.4 %), Micromonospora maritima DSM 45782T (99.3 %), Micromonospora sediminicola DSM 45794T (99.3 %), Micromonospora aurantiaca DSM 43813T (99.2 %) and Micromonospora chaiyaphumensis DSM 45246T (99.2 %). The results of OrthoANIu analysis showed the highest similarity to Micromonospora chalcea DSM 43026T (96.4 %). However, the 16S rRNA and gyrB gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38T as a novel species. On the basis of the results presented here, we propose to classify strain A38T (=LMG 30467T=CECT 30034T) as the type strain of the novel species Micromonospora fluminis sp. nov | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a OrthoANIu | |
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| 650 | 4 | |a polyphasic taxonomy | |
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| 650 | 7 | |a Fatty Acids |2 NLM | |
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| 700 | 1 | |a Wieme, Anneleen D |e verfasserin |4 aut | |
| 700 | 1 | |a Rodríguez Pérez, Suyén |e verfasserin |4 aut | |
| 700 | 1 | |a Llauradó Maury, Gabriel |e verfasserin |4 aut | |
| 700 | 1 | |a Peeters, Charlotte |e verfasserin |4 aut | |
| 700 | 1 | |a Snauwaert, Cindy |e verfasserin |4 aut | |
| 700 | 1 | |a Lescaylle Veranes, Yaneisy |e verfasserin |4 aut | |
| 700 | 1 | |a Peña Zamora, Leonor |e verfasserin |4 aut | |
| 700 | 1 | |a Schumann, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Vandamme, Peter A |e verfasserin |4 aut | |
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