A Novel Aminoacyl-tRNA Synthetase Appended Domain Can Supply the Core Synthetase with Its Amino Acid Substrate

The structural organization and functionality of aminoacyl-tRNA synthetases have been expanded through polypeptide additions to their core aminoacylation domain. We have identified a novel domain appended to the methionyl-tRNA synthetase (MetRS) of the intracellular pathogen Mycoplasma penetrans. Sequence analysis of this N-terminal region suggests the appended domain is an aminotransferase, which we demonstrate here. The aminotransferase domain of MpMetRS is capable of generating methionine from its α-keto acid analog, 2-keto-4-methylthiobutyrate (KMTB). The methionine thus produced can be subsequently attached to cognate tRNAMet in the MpMetRS aminoacylation domain. Genomic erosion in the Mycoplasma species has impaired many canonical biosynthetic pathways, causing them to rely on their host for numerous metabolites. It is still unclear if this bifunctional MetRS is a key part of pathogen life cycle or is a neutral consequence of the reductive evolution experienced by Mycoplasma species.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:11

Enthalten in:

Genes - 11(2020), 11 vom: 07. Nov.

Sprache:

Englisch

Beteiligte Personen:

Muraski, Marc [VerfasserIn]
Nilsson, Emil [VerfasserIn]
Weekley, Benjamin [VerfasserIn]
Sharma, Sandhya Bharti [VerfasserIn]
Alexander, Rebecca W [VerfasserIn]

Links:

Volltext

Themen:

2-keto-4-methylthiobutyric acid
583-92-6
9014-25-9
AE28F7PNPL
Amino Acids
Amino Acyl-tRNA Synthetases
Aminoacylation
Aminotransferase
Bifunctional
Channeling
EC 2.6.1.-
EC 6.1.1.-
EC 6.1.1.10
Journal Article
Methionine
Methionine-tRNA Ligase
Pyridoxal phosphate
RNA, Transfer
Research Support, Non-U.S. Gov't
TRNA
Transaminases

Anmerkungen:

Date Completed 26.07.2021

Date Revised 26.07.2021

published: Electronic

Citation Status MEDLINE

doi:

10.3390/genes11111320

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM317393596