TGF-β1-regulated miR-3691-3p targets E2F3 and PRDM1 to inhibit prostate cancer progression
Transforming growth factor-β1 (TGF-β1) acts as a tumor promoter in advanced prostate cancer (PCa). We speculated that microRNAs (miRNAs) that are inhibited by TGF-β1 might exert anti-tumor effects. To assess this, we identified several miRNAs downregulated by TGF-β1 in PCa cell lines and selected miR-3691-3p for detailed analysis as a candidate anti-oncogene miRNA. miR-3691-3p was expressed at significantly lower levels in human PCa tissue compared with paired benign prostatic hyperplasia tissue, and its expression level correlated inversely with aggressive clinical pathological features. Overexpression of miR-3691-3p in PCa cell lines inhibited proliferation, migration, and invasion, and promoted apoptosis. The miR-3691-3p target genes E2F transcription factor 3 (E2F3) and PR domain containing 1, with ZNF domain (PRDM1) were upregulated in miR-3691-3p-overexpressing PCa cells, and silencing of E2F3 or PRDM1 suppressed PCa cell proliferation, migration, and invasion. Treatment of mice bearing PCa xenografts with a miR-3691-3p agomir inhibited tumor growth and promoted tumor cell apoptosis. Consistent with the negative regulation of E2F3 and PRDM1 by miR-3691-3p, both proteins were overexpressed in clinical PCa specimens compared with noncancerous prostate tissue. Our results indicate that TGF-β1-regulated miR-3691-3p acts as an anti-oncogene in PCa by downregulating E2F3 and PRDM1. These results provide novel insights into the mechanisms by which TGF-β1 contributes to the progression of PCa.
| Errataetall: |
RetractionIn: Asian J Androl. 2022 Nov-Dec;24(6):684. - PMID 36254891 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
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| Enthalten in: |
Asian journal of andrology - 23(2021), 2 vom: 06. März, Seite 188-196 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hu, Yue-Mei [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 29.11.2021 Date Revised 30.12.2022 published: Print RetractionIn: Asian J Androl. 2022 Nov-Dec;24(6):684. - PMID 36254891 Citation Status MEDLINE |
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| doi: |
10.4103/aja.aja_60_20 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM31726821X |
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| 100 | 1 | |a Hu, Yue-Mei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a TGF-β1-regulated miR-3691-3p targets E2F3 and PRDM1 to inhibit prostate cancer progression |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 29.11.2021 | ||
| 500 | |a Date Revised 30.12.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a RetractionIn: Asian J Androl. 2022 Nov-Dec;24(6):684. - PMID 36254891 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Transforming growth factor-β1 (TGF-β1) acts as a tumor promoter in advanced prostate cancer (PCa). We speculated that microRNAs (miRNAs) that are inhibited by TGF-β1 might exert anti-tumor effects. To assess this, we identified several miRNAs downregulated by TGF-β1 in PCa cell lines and selected miR-3691-3p for detailed analysis as a candidate anti-oncogene miRNA. miR-3691-3p was expressed at significantly lower levels in human PCa tissue compared with paired benign prostatic hyperplasia tissue, and its expression level correlated inversely with aggressive clinical pathological features. Overexpression of miR-3691-3p in PCa cell lines inhibited proliferation, migration, and invasion, and promoted apoptosis. The miR-3691-3p target genes E2F transcription factor 3 (E2F3) and PR domain containing 1, with ZNF domain (PRDM1) were upregulated in miR-3691-3p-overexpressing PCa cells, and silencing of E2F3 or PRDM1 suppressed PCa cell proliferation, migration, and invasion. Treatment of mice bearing PCa xenografts with a miR-3691-3p agomir inhibited tumor growth and promoted tumor cell apoptosis. Consistent with the negative regulation of E2F3 and PRDM1 by miR-3691-3p, both proteins were overexpressed in clinical PCa specimens compared with noncancerous prostate tissue. Our results indicate that TGF-β1-regulated miR-3691-3p acts as an anti-oncogene in PCa by downregulating E2F3 and PRDM1. These results provide novel insights into the mechanisms by which TGF-β1 contributes to the progression of PCa | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Retracted Publication | |
| 650 | 4 | |a E2F transcription factor 3; miR-3691-3p; PR domain containing 1 | |
| 650 | 4 | |a with ZNF domain; prostate cancer; transforming growth factor-β1 | |
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| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a TGFB1 protein, human |2 NLM | |
| 650 | 7 | |a Transforming Growth Factor beta1 |2 NLM | |
| 650 | 7 | |a PRDM1 protein, human |2 NLM | |
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| 700 | 1 | |a Lou, Xiao-Li |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Bao-Zhu |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Wan, Shan |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Mao-Min |e verfasserin |4 aut | |
| 700 | 1 | |a Tao, Kun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yong-Sheng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shou-Li |e verfasserin |4 aut | |
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