Photodynamic inactivation of Streptococcus mutans by curcumin in combination with EDTA

Copyright © 2020 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved..

OBJECTIVE: This study aimed to test the efficacy of photodynamic inactivation (PDI) mediated by curcumin with EDTA against Streptococcus mutans in planktonic suspension using blue LED light.

METHODS: Antibacterial activity of curcumin and EDTA was evaluated by determination of their minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The fractional inhibitory concentration index (FICI) was used to estimate the synergistic effect of various combination ratios of curcumin and EDTA against S. mutans. Cultures of S. mutans (18 h, 37 °C, 5% C02) were prepared to test the effect of curcumin-mediated PDI (50 μM and 500 μM) with or without 0.4% EDTA and 40 s of light-activation with blue light. EDTA and each concentration of curcumin were also tested individually. Chlorhexidine (0.2%), was used as positive control. Planktonic suspensions were also analyzed by viable colony counts (VCC), confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and polymerase chain reaction (PCR).

RESULTS: The MIC values of curcumin and EDTA were 5 mM and 0.125% respectively. FICI showed a synergistic interaction between curcumin and EDTA. All the combinations with curcumin and blue LED light resulted in a complete inactivation of the S. mutans and CLSM confirms these results, TEM showed morphological changes produced by the PDI. No damage on DNA structure was detected by PCR.

SIGNIFICANCE: Curcumin-mediated PDI with EDTA using a blue light, shows a strong inhibitory effect against S. mutans in planktonic culture. Because of the unspecific target mechanism, it could be a promising technique for disinfection of dental tissues.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:37

Enthalten in:

Dental materials : official publication of the Academy of Dental Materials - 37(2021), 1 vom: 01. Jan., Seite e1-e14

Sprache:

Englisch

Beteiligte Personen:

Nima, Gabriel [VerfasserIn]
Soto-Montero, Jorge [VerfasserIn]
Alves, Lívia A [VerfasserIn]
Mattos-Graner, Renata O [VerfasserIn]
Giannini, Marcelo [VerfasserIn]

Links:

Volltext

Themen:

9G34HU7RV0
Curcumin
Dental curing lights
Disinfection
Edetic Acid
IT942ZTH98
Journal Article
Photodynamic therapy
Photosensitizing Agents
Photosensitizing agents
Research Support, Non-U.S. Gov't
Streptococcus mutans

Anmerkungen:

Date Completed 23.04.2021

Date Revised 23.04.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.dental.2020.09.015

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM317122029