Long non-coding RNA H19 deficiency ameliorates bleomycin-induced pulmonary inflammation and fibrosis
BACKGROUND: The poor understanding of pathogenesis in idiopathic pulmonary fibrosis (IPF) impaired development of effective therapeutic strategies. The aim of the current study is to investigate the roles of long non-coding RNA H19 (lncRNA H19) in the pulmonary inflammation and fibrosis of IPF.
METHODS: Bleomycin was used to induce pulmonary inflammation and fibrosis in mice. The mRNAs and proteins expression in lung tissues was determined by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. H19 knockout (H19-/-) mice were generated by CRISPR/Cas9.
RESULTS: The expression of H19 mRNA was up-regulated in fibrotic lungs patients with IPF as well as in lungs tissues that obtained from bleomycin-treated mice. H19-/- mice suppressed bleomycin-mediated pulmonary inflammation and inhibited the Il6/Stat3 signaling. H19 deficiency ameliorated bleomycin-induced pulmonary fibrosis and repressed the activation of TGF-β/Smad and S1pr2/Sphk2 in the lungs of bleomycin-treated mice.
CONCLUSIONS: Our data suggests that H19 is a profibrotic lncRNA and a potential therapeutic target for IPF.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
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| Enthalten in: |
Respiratory research - 21(2020), 1 vom: 02. Nov., Seite 290 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wan, Xiaoyu [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 15.09.2021 Date Revised 15.09.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1186/s12931-020-01534-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM317071548 |
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| 041 | |a eng | ||
| 100 | 1 | |a Wan, Xiaoyu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Long non-coding RNA H19 deficiency ameliorates bleomycin-induced pulmonary inflammation and fibrosis |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 15.09.2021 | ||
| 500 | |a Date Revised 15.09.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: The poor understanding of pathogenesis in idiopathic pulmonary fibrosis (IPF) impaired development of effective therapeutic strategies. The aim of the current study is to investigate the roles of long non-coding RNA H19 (lncRNA H19) in the pulmonary inflammation and fibrosis of IPF | ||
| 520 | |a METHODS: Bleomycin was used to induce pulmonary inflammation and fibrosis in mice. The mRNAs and proteins expression in lung tissues was determined by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. H19 knockout (H19-/-) mice were generated by CRISPR/Cas9 | ||
| 520 | |a RESULTS: The expression of H19 mRNA was up-regulated in fibrotic lungs patients with IPF as well as in lungs tissues that obtained from bleomycin-treated mice. H19-/- mice suppressed bleomycin-mediated pulmonary inflammation and inhibited the Il6/Stat3 signaling. H19 deficiency ameliorated bleomycin-induced pulmonary fibrosis and repressed the activation of TGF-β/Smad and S1pr2/Sphk2 in the lungs of bleomycin-treated mice | ||
| 520 | |a CONCLUSIONS: Our data suggests that H19 is a profibrotic lncRNA and a potential therapeutic target for IPF | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Pulmonary fibrosis | |
| 650 | 4 | |a S1pr2 | |
| 650 | 4 | |a Smad | |
| 650 | 4 | |a lncRNA H19 | |
| 650 | 7 | |a H19 long non-coding RNA |2 NLM | |
| 650 | 7 | |a Interleukin-6 |2 NLM | |
| 650 | 7 | |a RNA, Long Noncoding |2 NLM | |
| 650 | 7 | |a STAT3 Transcription Factor |2 NLM | |
| 650 | 7 | |a Smad Proteins, Receptor-Regulated |2 NLM | |
| 650 | 7 | |a Sphingosine-1-Phosphate Receptors |2 NLM | |
| 650 | 7 | |a Stat3 protein, mouse |2 NLM | |
| 650 | 7 | |a Tgfb1 protein, mouse |2 NLM | |
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| 650 | 7 | |a interleukin-6, mouse |2 NLM | |
| 650 | 7 | |a sphingosine-1-phosphate receptor-2, mouse |2 NLM | |
| 650 | 7 | |a Bleomycin |2 NLM | |
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| 650 | 7 | |a Phosphotransferases (Alcohol Group Acceptor) |2 NLM | |
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| 700 | 1 | |a Tian, Xinbei |e verfasserin |4 aut | |
| 700 | 1 | |a Du, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Yongtao |e verfasserin |4 aut | |
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