Evidence of positive regulation of mycobacteriophage D29 early gene expression obtained from an investigation using a temperature-sensitive mutant of the phage

© The Author(s) 2020. Published by Oxford University Press on behalf of FEMS..

Mycobacteriophages are phages that infect and kill Mycobacteria, several of which, Mycobacterium tuberculosis (Mtb), for example, cause the disease tuberculosis. Although genomes of many such phages have been sequenced, we have very little insight into how they express their genes in a controlled manner. To address this issue, we have raised a temperature-sensitive (ts) mutant of phage D29 that can grow at 37°C but not at 42°C and used it to perform differential gene expression and proteome analysis studies. Our analysis results indicate that expression of genes located in the right arm, considered to be early expressed, was lowered as the temperature was shifted from 37°C to 42°C. In contrast, expression of those on the left, the late genes were only marginally affected. Thus, we conclude that transcription of genes from the two arms takes place independently of each other and that a specific factor must be controlling the expression of the right arm genes. We also observe that within the right arm itself; there exists a mechanism to ensure high-level synthesis of Gp48, a thymidylate synthase X. Enhanced presence of this protein in infected cells results in delayed lysis and higher phage yields.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:367

Enthalten in:

FEMS microbiology letters - 367(2020), 21 vom: 23. Nov.

Sprache:

Englisch

Beteiligte Personen:

Ghosh, Shrestha [VerfasserIn]
Shaw, Rahul [VerfasserIn]
Sarkar, Apurba [VerfasserIn]
Gupta, Sujoy K Das [VerfasserIn]

Links:

Volltext

Themen:

Journal Article
Mycobacteriophage
Promoters
Proteomics
Research Support, Non-U.S. Gov't
Temperature-sensitive mutants
Transcriptomics

Anmerkungen:

Date Completed 14.07.2021

Date Revised 14.07.2021

published: Print

Citation Status MEDLINE

doi:

10.1093/femsle/fnaa176

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM316876461