Molecular Design and Production of AAV Viral Vectors for Gene Therapy
Adeno-associated virus (AAV) is a helper-dependent single-stranded DNA parvovirus. Over the years, AAV has become the vector of choice in the gene therapy field due to its safety profile and low immunogenicity. With a carrying capacity of 4.2 kbp, these vectors have demonstrated their clinical value, especially in the field of ophthalmology. Herein we describe methods for the molecular design and packaging of AAV viral vectors. These methods apply to the design of single-stranded or self-complementary AAV vectors.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2225 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2225(2021) vom: 27., Seite 77-92 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ridley, Raela B [VerfasserIn] |
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| Links: |
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| Themen: |
AAV |
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| Anmerkungen: |
Date Completed 26.03.2021 Date Revised 26.03.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-1012-1_5 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM316773271 |
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| 245 | 1 | 0 | |a Molecular Design and Production of AAV Viral Vectors for Gene Therapy |
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| 500 | |a Date Revised 26.03.2021 | ||
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Adeno-associated virus (AAV) is a helper-dependent single-stranded DNA parvovirus. Over the years, AAV has become the vector of choice in the gene therapy field due to its safety profile and low immunogenicity. With a carrying capacity of 4.2 kbp, these vectors have demonstrated their clinical value, especially in the field of ophthalmology. Herein we describe methods for the molecular design and packaging of AAV viral vectors. These methods apply to the design of single-stranded or self-complementary AAV vectors | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a AAV | |
| 650 | 4 | |a Cloning | |
| 650 | 4 | |a Gene therapy | |
| 650 | 4 | |a Ligation | |
| 650 | 4 | |a PCR | |
| 650 | 4 | |a Transformation | |
| 650 | 4 | |a Transgene | |
| 650 | 7 | |a DNA Primers |2 NLM | |
| 650 | 7 | |a DNA, Single-Stranded |2 NLM | |
| 700 | 1 | |a Walsh, Erin M |e verfasserin |4 aut | |
| 700 | 1 | |a Ildefonso, Cristhian J |e verfasserin |4 aut | |
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