Carbon as a Simple Support for Redox Biocatalysis in Continuous Flow
A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, providing in situ cofactor recycling. Other metrics were also considered as optimization targets, such as low E factors between 2.5-11 and space-time yields of up to 22.9 g L-1 h-1. The long-term stability of the biocatalytic reactor was also demonstrated, with full conversion maintained over more than 30 h of continuous operation.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
|---|---|
| Enthalten in: |
Organic process research & development - 24(2020), 10 vom: 16. Okt., Seite 2281-2287 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Poznansky, Barnabas [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Revised 29.11.2021 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1021/acs.oprd.9b00410 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM316696374 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM316696374 | ||
| 003 | DE-627 | ||
| 005 | 20231225161639.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/acs.oprd.9b00410 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1055.xml |
| 035 | |a (DE-627)NLM316696374 | ||
| 035 | |a (NLM)33100814 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Poznansky, Barnabas |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Carbon as a Simple Support for Redox Biocatalysis in Continuous Flow |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 29.11.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, providing in situ cofactor recycling. Other metrics were also considered as optimization targets, such as low E factors between 2.5-11 and space-time yields of up to 22.9 g L-1 h-1. The long-term stability of the biocatalytic reactor was also demonstrated, with full conversion maintained over more than 30 h of continuous operation | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Thompson, Lisa A |e verfasserin |4 aut | |
| 700 | 1 | |a Warren, Sarah A |e verfasserin |4 aut | |
| 700 | 1 | |a Reeve, Holly A |e verfasserin |4 aut | |
| 700 | 1 | |a Vincent, Kylie A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Organic process research & development |d 1999 |g 24(2020), 10 vom: 16. Okt., Seite 2281-2287 |w (DE-627)NLM098256874 |x 1083-6160 |7 nnns |
| 773 | 1 | 8 | |g volume:24 |g year:2020 |g number:10 |g day:16 |g month:10 |g pages:2281-2287 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1021/acs.oprd.9b00410 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 24 |j 2020 |e 10 |b 16 |c 10 |h 2281-2287 | ||