Involvement of boron transporter BOR1 in growth under low boron and high nitrate conditions in Arabidopsis thaliana
© 2020 Scandinavian Plant Physiology Society..
BOR1 is an efflux transporter of boron (B), responsible for loading B into the xylem. It has been reported that nitrate (NO3 - ) concentrations significantly influence B concentrations in leaves and BOR1 mRNA accumulation in roots. Here, to unravel the interactive effects of B and NO3 - on plant growth and the function of BOR1 under the combination of B and NO3 - , seedling growth was analyzed in Col-0 and bor1 mutants. The growth of bor1 mutants was negatively affected by high NO3 - but neither by potassium chloride (KCl) nor ammonium (NH4 + ) under low B conditions, suggesting the involvement of BOR1 in growth under high NO3 - . Mutants of bor2 and bor4 did not exhibit such growth responses, suggesting that this effect was specific to BOR1 among the BORs tested. Under low B conditions, loss of the BOR1 function led to a more significant decrease in B concentrations in the presence of high NO3 - compared to normal NO3 - . Additionally, grafting experiments demonstrated that these effects of NO3 - occurred when BOR1 is absent in roots. High NO3 - treatment elevated BOR1 mRNA accumulation while the BOR1 protein accumulation was downregulated. These apparent opposite responses indicated that the transcriptional and (post-)translational regulations follow different patterns. Our work provides evidence of a novel regulation of BOR1 and another B transport system by both B and NO3 - in an interactive manner.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:171 |
|---|---|
| Enthalten in: |
Physiologia plantarum - 171(2021), 4 vom: 08. Apr., Seite 703-713 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Qing [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antiporters |
|---|
| Anmerkungen: |
Date Completed 31.03.2021 Date Revised 31.05.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/ppl.13249 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM316594067 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM316594067 | ||
| 003 | DE-627 | ||
| 005 | 20231225161427.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/ppl.13249 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1055.xml |
| 035 | |a (DE-627)NLM316594067 | ||
| 035 | |a (NLM)33090485 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Qing |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Involvement of boron transporter BOR1 in growth under low boron and high nitrate conditions in Arabidopsis thaliana |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 31.03.2021 | ||
| 500 | |a Date Revised 31.05.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 Scandinavian Plant Physiology Society. | ||
| 520 | |a BOR1 is an efflux transporter of boron (B), responsible for loading B into the xylem. It has been reported that nitrate (NO3 - ) concentrations significantly influence B concentrations in leaves and BOR1 mRNA accumulation in roots. Here, to unravel the interactive effects of B and NO3 - on plant growth and the function of BOR1 under the combination of B and NO3 - , seedling growth was analyzed in Col-0 and bor1 mutants. The growth of bor1 mutants was negatively affected by high NO3 - but neither by potassium chloride (KCl) nor ammonium (NH4 + ) under low B conditions, suggesting the involvement of BOR1 in growth under high NO3 - . Mutants of bor2 and bor4 did not exhibit such growth responses, suggesting that this effect was specific to BOR1 among the BORs tested. Under low B conditions, loss of the BOR1 function led to a more significant decrease in B concentrations in the presence of high NO3 - compared to normal NO3 - . Additionally, grafting experiments demonstrated that these effects of NO3 - occurred when BOR1 is absent in roots. High NO3 - treatment elevated BOR1 mRNA accumulation while the BOR1 protein accumulation was downregulated. These apparent opposite responses indicated that the transcriptional and (post-)translational regulations follow different patterns. Our work provides evidence of a novel regulation of BOR1 and another B transport system by both B and NO3 - in an interactive manner | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Antiporters |2 NLM | |
| 650 | 7 | |a Arabidopsis Proteins |2 NLM | |
| 650 | 7 | |a BOR1 protein, Arabidopsis |2 NLM | |
| 650 | 7 | |a Nitrates |2 NLM | |
| 650 | 7 | |a Boron |2 NLM | |
| 650 | 7 | |a N9E3X5056Q |2 NLM | |
| 700 | 1 | |a Zhang, Wenna |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Hua |e verfasserin |4 aut | |
| 700 | 1 | |a Sotta, Naoyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Beier, Marcel P |e verfasserin |4 aut | |
| 700 | 1 | |a Takano, Junpei |e verfasserin |4 aut | |
| 700 | 1 | |a Miwa, Kyoko |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Lihong |e verfasserin |4 aut | |
| 700 | 1 | |a Fujiwara, Toru |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Physiologia plantarum |d 1969 |g 171(2021), 4 vom: 08. Apr., Seite 703-713 |w (DE-627)NLM012364339 |x 1399-3054 |7 nnns |
| 773 | 1 | 8 | |g volume:171 |g year:2021 |g number:4 |g day:08 |g month:04 |g pages:703-713 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/ppl.13249 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 171 |j 2021 |e 4 |b 08 |c 04 |h 703-713 | ||