Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19). The spike protein expressed on the surface of this virus is highly glycosylated and plays an essential role during the process of infection. We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry. The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein were characterized using this approach. Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins. The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species. In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells. These results provide useful information for the study of the interactions of SARS-CoV-2 viral proteins and for the development of effective vaccines and therapeutics.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:92

Enthalten in:

Analytical chemistry - 92(2020), 21 vom: 03. Nov., Seite 14730-14739

Sprache:

Englisch

Beteiligte Personen:

Wang, Dongxia [VerfasserIn]
Baudys, Jakub [VerfasserIn]
Bundy, Jonathan L [VerfasserIn]
Solano, Maria [VerfasserIn]
Keppel, Theodore [VerfasserIn]
Barr, John R [VerfasserIn]

Links:

Volltext

Themen:

Journal Article
Polysaccharides
Spike Glycoprotein, Coronavirus
Spike protein, SARS-CoV-2

Anmerkungen:

Date Completed 17.11.2020

Date Revised 29.08.2023

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1021/acs.analchem.0c03301

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM31633930X